Meyer Joana B, Trapiello Estefanía, Senn-Irlet Beatrice, Sieber Thomas N, Cornejo Carolina, Aghayeva Dilzara, González Ana J, Prospero Simone
Swiss Federal Institute for Forest, Snow and Landscape Research WSL, CH-8903 Birmensdorf, Switzerland.
Servicio Regional de Investigacion y Desarrollo Agroalimentario (SERIDA), Principado de Asturias, 33300 Villaviciosa, Spain; Department of Engineering and Agricultural Sciences, Area of Crop Production, University of León, Avda. Portugal 41, 24071 León, Spain.
Fungal Biol. 2017 Aug;121(8):625-637. doi: 10.1016/j.funbio.2017.04.001. Epub 2017 Apr 27.
In this paper we resolve the taxonomic status of the fungus Diplodina castaneae (Ascomycetes, Diaporthales, Gnomoniaceae) which occurs on the European chestnut (Castanea sativa) as endophyte and as the causal agent of Javart disease. Specimens from Switzerland, Spain, and Azerbaijan were sequenced at five nuclear loci (β-tubulin, EF-1α, ITS, LSU, and RPB2). Phylogenies were inferred to place D. castaneae in the Gnomoniaceae family. Moreover, growth rates and morphological characteristics on different agar media were assessed and compared to those of Gnomoniopsis castaneae, which can easily be confused with D. castaneae. Based on morphological and phylogenetic characteristics, we propose to reallocate D. castaneae to the genus Sirococcus, as S. castaneae comb. nov.
在本文中，我们确定了真菌栗生双壳菌（子囊菌门，座囊菌目，日规壳科）的分类地位，该真菌作为内生菌存在于欧洲栗（Castanea sativa）上，并作为贾瓦特病的病原体。对来自瑞士、西班牙和阿塞拜疆的标本在五个核基因座（β-微管蛋白、EF-1α、ITS、LSU和RPB2）进行了测序。通过系统发育分析将栗生双壳菌置于日规壳科中。此外，评估了其在不同琼脂培养基上的生长速率和形态特征，并与容易与栗生双壳菌混淆的栗生日规壳菌的生长速率和形态特征进行了比较。基于形态学和系统发育特征，我们建议将栗生双壳菌重新归类到球座菌属，即新组合种栗球座菌（S. castaneae comb. nov.） 。
