Two plant species, and , are cultivated in China to produce chestnut crops. Leaf spot diseases commonly occur in plantations, however, little is known about the fungal species associated with chestnut leaf spots. In this study, leaf samples of and were collected from Beijing, Guizhou, Hunan, Sichuan and Yunnan Provinces, and leaf-inhabiting fungi were identified based on morphology and phylogeny. As a result, twenty-six fungal species were confirmed, including one new family, one new genus, and five new species. The new taxa are Pyrisporaceae fam. nov., gen. nov., sp. nov., sp. nov., sp. nov., sp. nov. and sp. nov.