帕金森病的诊断与氧化 DJ-1 蛋白水平。

Diagnosis of Parkinson's disease and the level of oxidized DJ-1 protein.

机构信息

Department of Neurology, Faculty of Medicine, Kindai University, 377-2 Ohno-Higashi, Osaka-Sayama, Osaka 589-8511, Japan.

Department of Neurology, Faculty of Medicine, Kindai University, 377-2 Ohno-Higashi, Osaka-Sayama, Osaka 589-8511, Japan; Department of Life Science, Faculty of Science and Engineering, Kindai University, 3-4-1 Kowakae, Higashiosaka, Osaka 577-8502, Japan.

出版信息

Neurosci Res. 2018 Mar;128:58-62. doi: 10.1016/j.neures.2017.06.008. Epub 2017 Jul 10.

DOI:10.1016/j.neures.2017.06.008

PMID:28705587

Abstract

Parkinson's disease (PD) is difficult to distinguish from progressive supranuclear palsy (PSP) and multiple system atrophy (MSA); in addition, biomarker studies in PD mostly focused on those found in the cerebrospinal fluid, and there are few reports of simple biomarkers identified by blood analysis. Previously, the DJ-1 gene was identified as a causative gene of familial PD. Oxidized DJ-1 protein (oxDJ-1) levels were reported to increase in the blood of patients with unmedicated PD. Therefore, we determined the levels of oxDJ-1 in the erythrocytes of patients with PD, PSP, and MSA using ELISA. The oxDJ-1 levels were 165±117, 96±78, and 69±40ng/mg protein in the PD, PSP, and MSA groups, respectively. The mean level in disease control group was 66±31, revealing significant differences between the PD and PSP groups, the PD and MSA groups, and the PD and disease control groups. Our results indicated that oxDJ-1 levels in erythrocytes can be used as a marker for the differential diagnosis of PD.

摘要

帕金森病（PD）与进行性核上性麻痹（PSP）和多系统萎缩（MSA）难以区分；此外，PD 的生物标志物研究主要集中在脑脊液中发现的标志物上，很少有通过血液分析确定的简单生物标志物的报道。先前，DJ-1 基因被确定为家族性 PD 的致病基因。据报道，未经治疗的 PD 患者血液中的氧化 DJ-1 蛋白（oxDJ-1）水平升高。因此，我们使用 ELISA 法测定了 PD、PSP 和 MSA 患者红细胞中的 oxDJ-1 水平。PD、PSP 和 MSA 组的 oxDJ-1 水平分别为 165±117、96±78 和 69±40ng/mg 蛋白。疾病对照组的平均值为 66±31，PD 与 PSP 组、PD 与 MSA 组和 PD 与疾病对照组之间存在显著差异。我们的结果表明，红细胞中的 oxDJ-1 水平可作为 PD 鉴别诊断的标志物。