Ogawa Ikuko, Saito Yoshiro, Saigoh Kazumasa, Hosoi Yoshie, Mitsui Yoshiyuki, Noguchi Noriko, Kusunoki Susumu
Department of Neurology, Kinki University Faculty of Medicine.
Brain Nerve. 2014 Apr;66(4):471-7.
The search for biomarkers of Parkinson's disease (PD) typically focuses on cerebrospinal fluid components, with very few reports on simple biomarkers identifiable by blood analysis. In this report, we determined the level of oxidized DJ-1 protein (oxDJ-1) in red blood cells by ELISA and examined the association with MIBG myocardial scintigraphy. Levels of oxDJ-1 were higher in unmedicated patients with PD (142.2 ± 21.8 ng oxDJ-1/mg protein; n = 13) compared to the L-DOPA-treated group (85.6 ± 10.1 ng oxDJ-1/mg protein; n = 10) and controls (56.0 ± 6.2 ng oxDJ-1/mg protein; n = 17), thereby showing significant intergroup differences. Intervention with L-DOPA showed a tendency to decrease oxDJ-1 levels in patients. The diagnostic sensitivity of oxDJ-1 measurement was 87% and that of simultaneously conducted MIBG scintigraphy was 89%; this showed that the diagnostic sensitivity was comparable. Our results showed that measurement of oxDJ-1 levels in red blood cells can be useful and oxDJ-1 can be used as a biomarker for the early diagnosis of PD. (Receieved July 31, 2013; Accepted October 16, 2013; Published April 1, 2014).
对帕金森病（PD）生物标志物的研究通常聚焦于脑脊液成分，而关于通过血液分析可识别的简单生物标志物的报道极少。在本报告中，我们通过酶联免疫吸附测定法（ELISA）测定了红细胞中氧化型DJ-1蛋白（oxDJ-1）的水平，并研究了其与间碘苄胍（MIBG）心肌闪烁显像的相关性。与左旋多巴治疗组（85.6±10.1 ng oxDJ-1/毫克蛋白；n = 10）和对照组（56.0±6.2 ng oxDJ-1/毫克蛋白；n = 17）相比，未接受药物治疗的PD患者的oxDJ-1水平更高（142.2±21.8 ng oxDJ-1/毫克蛋白；n = 13），从而显示出显著的组间差异。左旋多巴干预显示患者的oxDJ-1水平有降低趋势。oxDJ-1测量的诊断敏感性为87%，同时进行的MIBG闪烁显像的诊断敏感性为89%；这表明诊断敏感性相当。我们的结果表明，测定红细胞中的oxDJ-1水平可能有用，且oxDJ-1可作为PD早期诊断的生物标志物。（2013年7月31日收到；2013年10月16日接受；2014年4月1日发表）
