Tianjin Key Laboratory of Cardiovascular Remodeling & Target Organ Injury, Pingjin Hospital Heart Center, Tianjin 300162, China; Central Laboratory, Logistics University of Chinese People's Armed Police Force, Tianjin 300309, China.
Central Laboratory, Logistics University of Chinese People's Armed Police Force, Tianjin 300309, China.
Biochim Biophys Acta Proteins Proteom. 2017 Sep;1865(9):1207-1214. doi: 10.1016/j.bbapap.2017.07.003. Epub 2017 Jul 10.
Hypertensive disorder in pregnancy (HDP) refers to a series of diseases that cause the hypertension during pregnancy, including HDP, preeclampsia (PE) and eclampsia. This study screens differentially expressed proteins of placenta tissues in PE cases using 2D LC-MS/MS quantitative proteomics strategy. A total of 2281 proteins are quantified, of these, 145 altering expression proteins are successfully screened between PE and control cases (p<0.05). Bioinformatics analysis suggests that these proteins are mainly involved in many biological processes, such as oxidation reduction, mitochondrion organization, and acute inflammatory response. Especially, the glutamine metabolic process related molecules, GPX1, GPX3, SMS, GGCT, GSTK1, NFκB, GSTT2, SOD1 and GCLM, are involved in the switching process from oxidized glutathione (GSSG) conversion to the reduced glutathione (GSH) by glutathione, mercapturic acid and arginine metabolism process. Results of this study revealed that glutathione metabolism disorder of placenta tissues may contribute to the occurrence of PE disease.
妊娠期高血压疾病（HDP）是指妊娠期发生的一系列高血压疾病，包括 HDP、子痫前期（PE）和子痫。本研究采用 2D LC-MS/MS 定量蛋白质组学策略筛选 PE 病例胎盘组织中的差异表达蛋白。共定量了 2281 种蛋白质，其中成功筛选出 PE 病例与对照组之间表达改变的 145 种蛋白质（p<0.05）。生物信息学分析表明，这些蛋白质主要参与许多生物学过程，如氧化还原、线粒体组织和急性炎症反应。特别是与谷氨酰胺代谢过程相关的分子，如 GPX1、GPX3、SMS、GGCT、GSTK1、NFκB、GSTT2、SOD1 和 GCLM，参与了谷胱甘肽、硫氨酸和精氨酸代谢过程中氧化型谷胱甘肽（GSSG）转化为还原型谷胱甘肽（GSH）的转换过程。本研究结果表明，胎盘组织谷胱甘肽代谢紊乱可能导致 PE 疾病的发生。
