Clinical Medical Research Center, Guangdong Provincial Engineering Research Center of Autoimmune Disease Precision Medicine, Shenzhen Engineering Research Center of Autoimmune Disease, The Second Clinical Medical College of Jinan University, The First Affiliated Hospital of Southern University of Science and Technology, Shenzhen People's Hospital, Shenzhen, China.
Guangxi Key Laboratory of Metabolic Disease Research, Nephrology Department, 924st Hospital, Guilin, China.
J Cell Mol Med. 2021 Nov;25(22):10614-10626. doi: 10.1111/jcmm.16997. Epub 2021 Oct 26.
Preeclampsia (PE) is a dangerous hypertensive disorder that occurs during pregnancy. The specific aetiology and pathogenesis of PE have yet to be clarified. To better reveal the specific pathogenesis of PE, we characterized the proteome and acetyl proteome (acetylome) profile of placental tissue from PE and normal-term pregnancy by label-free quantification proteomics technology and PRM analysis. In this research, 373 differentially expressed proteins (DEPs) were identified by proteome analysis. Functional enrichment analysis revealed significant enrichment of DEPs related to angiogenesis and the immune system. COL12A1, C4BPA and F13A1 may be potential biomarkers for PE diagnosis and new therapeutic targets. Additionally, 700 Kac sites were identified on 585 differentially acetylated proteins (DAPs) by acetylome analyses. These DAPs may participate in the occurrence and development of PE by affecting the complement and coagulation cascades pathway, which may have important implications for better understand the pathogenesis of PE. In conclusion, this study systematically analysed the reveals critical features of placental proteins in pregnant women with PE, providing a resource for exploring the contribution of lysine acetylation modification to PE.
子痫前期（PE）是一种发生在妊娠期间的危险高血压疾病。PE 的具体病因和发病机制尚未阐明。为了更好地揭示 PE 的具体发病机制，我们采用无标记定量蛋白质组学技术和 PRM 分析方法，对 PE 和足月妊娠的胎盘组织的蛋白质组和乙酰化蛋白质组（乙酰组）图谱进行了特征描述。在这项研究中，通过蛋白质组分析鉴定出 373 个差异表达蛋白（DEPs）。功能富集分析显示，DEPs 与血管生成和免疫系统相关的功能显著富集。COL12A1、C4BPA 和 F13A1 可能是 PE 诊断的潜在生物标志物和新的治疗靶点。此外，通过乙酰组分析鉴定出 585 个差异乙酰化蛋白（DAPs）上的 700 个 Kac 位点。这些 DAPs 可能通过影响补体和凝血级联途径参与 PE 的发生和发展，这对于更好地理解 PE 的发病机制具有重要意义。总之，本研究系统分析了患有 PE 的孕妇胎盘蛋白的关键特征，为探索赖氨酸乙酰化修饰对 PE 的贡献提供了资源。
