Kumar S A, Beach T A, Dickerman H W
Proc Natl Acad Sci U S A. 1979 May;76(5):2199-203. doi: 10.1073/pnas.76.5.2199.
The binding of estradiol--receptor complexes of mouse uterine cytosol to oligodeoxynucleotide celluloses is inhibited by the sulfonated polyaromatic dye Cibacron blue F3GA. The dye does not have any effect on the estradiol binding site. Additon of the dye to preformed estradiol--receptor--oligo(dT)-cellulose complex results in the release of estradiol--receptor. The inhibition of binding is competitive with respect to oligo(dT)- or oligo(dA)-Celluloses, suggesting that the effect of the dye is directly on the polynucleotide binding site of the receptor. The observed inhibitory effect of Cibacron blue is not a simple electrostatic effect of polyanions because heparin and polyglutamic acid are much less effective. The selective inhibition by Cibacron blue suggests that the polynucleotide binding domain of estradiol--receptor possesses a special "supersecondary" structure [Stellwagen, E. (1977) Acc. Chem. Res. 10, 92--98].
磺酸化多环芳烃染料汽巴蓝F3GA可抑制小鼠子宫胞质溶胶中的雌二醇-受体复合物与寡聚脱氧核苷酸纤维素的结合。该染料对雌二醇结合位点没有任何影响。将染料添加到预先形成的雌二醇-受体-寡聚（dT）-纤维素复合物中会导致雌二醇-受体的释放。结合抑制在寡聚（dT）-或寡聚（dA）-纤维素方面具有竞争性，这表明染料的作用直接作用于受体的多核苷酸结合位点。观察到的汽巴蓝的抑制作用不是聚阴离子的简单静电作用，因为肝素和聚谷氨酸的效果要差得多。汽巴蓝的选择性抑制表明雌二醇-受体的多核苷酸结合结构域具有特殊的“超二级”结构[斯特尔瓦根，E.（1977年）《化学研究述评》10，92 - 98]。
