环磷酸腺苷受体：在环磷酸腺苷存在的情况下经蛋白水解后环磷酸腺苷依赖性DNA结合活性丧失。
Cyclic adenosine monophosphate receptor: loss of cAMP-dependent DNA binding activity after proteolysis in the presence of cyclic adenosine monophosphate.
作者信息
Krakow J S, Pastan I
出版信息
Proc Natl Acad Sci U S A. 1973 Sep;70(9):2529-33. doi: 10.1073/pnas.70.9.2529.
Abstract
The cAMP receptor requires cAMP for DNA binding at pH 8.0 but shows cAMP-independent DNA binding at pH 6.0. Incubation of the cAMP receptor with proteolytic enzymes in the presence of cAMP results in loss of DNA-binding ability at pH 8, while it is still able to bind cAMP and DNA at pH 6. Incubation with proteolytic enzyme in the absence of cAMP does not affect the DNA-binding properties of the cAMP receptor. After proteolysis in the presence of cAMP, analysis by sodium dodecyl sulfateacrylamide-gel electrophoresis shows that the 22,500-dalton subunit characteristic of the untreated protein has been completely replaced by a 12,500-dalton fragment.
摘要
环磷酸腺苷（cAMP）受体在pH 8.0时需要cAMP才能结合DNA，但在pH 6.0时表现出不依赖cAMP的DNA结合能力。在cAMP存在的情况下，用蛋白水解酶孵育cAMP受体，会导致其在pH 8时失去DNA结合能力，而在pH 6时仍能结合cAMP和DNA。在没有cAMP的情况下用蛋白水解酶孵育，不会影响cAMP受体的DNA结合特性。在cAMP存在下进行蛋白水解后，通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析表明，未处理蛋白质特有的22,500道尔顿亚基已完全被一个12,500道尔顿的片段取代。
相似文献
1
Cyclic adenosine monophosphate receptor: loss of cAMP-dependent DNA binding activity after proteolysis in the presence of cyclic adenosine monophosphate.环磷酸腺苷受体：在环磷酸腺苷存在的情况下经蛋白水解后环磷酸腺苷依赖性DNA结合活性丧失。
Proc Natl Acad Sci U S A. 1973 Sep;70(9):2529-33. doi: 10.1073/pnas.70.9.2529.
2
Cyclic adenosine monophosphate receptor: effect of cyclic AMP analogues on DNA binding and proteolytic inactivation.环磷酸腺苷受体：环磷酸腺苷类似物对DNA结合及蛋白水解失活的影响
Biochim Biophys Acta. 1975 Apr 2;383(4):345-50. doi: 10.1016/0005-2787(75)90303-2.
3
Adenosine 3':5'-cyclic monophosphate-binding proteins in bovine and rat tissues.牛和大鼠组织中的3':5'-环磷酸腺苷结合蛋白
Biochem J. 1976 Nov;159(2):423-37. doi: 10.1042/bj1590423.
4
An equilibrium study of the cooperative binding of adenosine cyclic 3',5'-monophosphate and guanosine cyclic 3',5'-monophosphate to the adenosine cyclic 3',5'-monophosphate receptor protein from Escherichia coli.3',5'-环磷酸腺苷（cAMP）与3',5'-环磷酸鸟苷（cGMP）协同结合大肠杆菌3',5'-环磷酸腺苷受体蛋白的平衡研究。
Biochemistry. 1980 Oct 28;19(22):5124-30. doi: 10.1021/bi00563a029.
5
Effect of deoxyribopolymers and ribopolymers on the sensitivity of the cyclic-AMP receptor protein of Escherichia coli to proteolytic attack.脱氧核糖聚合物和核糖聚合物对大肠杆菌环磷酸腺苷受体蛋白对蛋白水解攻击敏感性的影响。
Arch Biochem Biophys. 1985 Jan;236(1):11-6. doi: 10.1016/0003-9861(85)90600-9.
6
Cross-linking of the cAMP receptor protein of Escherichia coli by o-phenylenedimaleimide as a probe of conformation.
Biochemistry. 1979 Apr 17;18(8):1519-25. doi: 10.1021/bi00575a020.
7
Absence of high-affinity adenosine 3',5'-monophosphate binding sites from the cytosol of three hepatic-derived cell lines.三种肝源性细胞系的胞质溶胶中缺乏高亲和力的3',5'-环磷酸腺苷结合位点。
Arch Biochem Biophys. 1974 Nov;165(1):359-68. doi: 10.1016/0003-9861(74)90174-x.
8
9
Purification of the cAMP receptor protein by affinity chromatography.通过亲和层析法纯化环磷酸腺苷受体蛋白。
Biochem Biophys Res Commun. 1974 Jul 24;59(2):813-21. doi: 10.1016/s0006-291x(74)80052-5.
引用本文的文献
1
Nitrogen assimilation in Escherichia coli: putting molecular data into a systems perspective.大肠杆菌中的氮同化：将分子数据置于系统视角下。
Microbiol Mol Biol Rev. 2013 Dec;77(4):628-95. doi: 10.1128/MMBR.00025-13.
2
CbpA: a polarly localized novel cyclic AMP-binding protein in Pseudomonas aeruginosa.CbpA：铜绿假单胞菌中一种极性定位的新型环磷酸腺苷结合蛋白。
J Bacteriol. 2009 Dec;191(23):7193-205. doi: 10.1128/JB.00970-09. Epub 2009 Oct 2.
3
The Mycobacterium bovis BCG cyclic AMP receptor-like protein is a functional DNA binding protein in vitro and in vivo, but its activity differs from that of its M. tuberculosis ortholog, Rv3676.牛分枝杆菌卡介苗环磷酸腺苷受体样蛋白在体外和体内均为功能性DNA结合蛋白，但其活性与其结核分枝杆菌直系同源蛋白Rv3676不同。
Infect Immun. 2007 Nov;75(11):5509-17. doi: 10.1128/IAI.00658-07. Epub 2007 Sep 4.
4
Characterization of Mycobacterium tuberculosis Rv3676 (CRPMt), a cyclic AMP receptor protein-like DNA binding protein.结核分枝杆菌Rv3676（CRPMt）的特性，一种环磷酸腺苷受体蛋白样DNA结合蛋白。
J Bacteriol. 2005 Nov;187(22):7795-804. doi: 10.1128/JB.187.22.7795-7804.2005.
5
Mapping cyclic nucleotide-induced conformational changes in cyclicAMP receptor protein by a protein footprinting technique using different chemical proteases.运用不同化学蛋白酶的蛋白质足迹技术绘制环磷酸腺苷受体蛋白中环核苷酸诱导的构象变化图谱。
Protein Sci. 1999 Mar;8(3):518-28. doi: 10.1110/ps.8.3.518.
6
Mechanism of CRP-mediated cya suppression in Escherichia coli.大肠杆菌中CRP介导的cya抑制机制。
J Bacteriol. 1983 Jan;153(1):191-9. doi: 10.1128/jb.153.1.191-199.1983.
7
Cloning and sequence of the crp gene of Escherichia coli K 12.大肠杆菌K12 crp基因的克隆与序列分析
Nucleic Acids Res. 1982 Feb 25;10(4):1363-78. doi: 10.1093/nar/10.4.1363.
8
Molecular cloning and nucleotide sequencing of the gene for E. coli cAMP receptor protein.大肠杆菌环磷酸腺苷受体蛋白基因的分子克隆与核苷酸测序
Nucleic Acids Res. 1982 Feb 25;10(4):1345-61. doi: 10.1093/nar/10.4.1345.
9
Mechanism for transcriptional action of cyclic AMP in Escherichia coli: entry into DNA to disrupt DNA secondary structure.环磷酸腺苷在大肠杆菌中转录作用的机制：进入DNA以破坏DNA二级结构。
Proc Natl Acad Sci U S A. 1981 Jul;78(7):4011-5. doi: 10.1073/pnas.78.7.4011.
10
Homologies between different procaryotic DNA-binding regulatory proteins and between their sites of action.不同原核生物DNA结合调节蛋白之间及其作用位点之间的同源性。
EMBO J. 1982;1(5):591-5. doi: 10.1002/j.1460-2075.1982.tb01213.x.
本文引用的文献
1
HELIX--RANDOM COIL TRANSITIONS IN DNA HOMOPOLYMER PAIRS.DNA同聚物对中的螺旋-无规卷曲转变
J Mol Biol. 1964 Apr;8:452-69. doi: 10.1016/s0022-2836(64)80003-6.
2
Novel method for measuring polyuridylic acid binding to ribosomes.测量多聚尿苷酸与核糖体结合的新方法。
Biochim Biophys Acta. 1970 Feb 18;199(2):447-52. doi: 10.1016/0005-2787(70)90087-0.
3
Studies with DNA-cellulose chromatography. I. DNA-binding proteins from Escherichia coli.DNA-纤维素色谱法研究。I. 来自大肠杆菌的DNA结合蛋白。
Cold Spring Harb Symp Quant Biol. 1968;33:289-305. doi: 10.1101/sqb.1968.033.01.033.
4
Enzymatic synthesis of deoxyribonucleic acid. XXVI. Physical and chemical studies of a homogeneous deoxyribonucleic acid polymerase.脱氧核糖核酸的酶促合成。二十六。一种纯一的脱氧核糖核酸聚合酶的物理和化学研究。
J Biol Chem. 1969 Jun 10;244(11):2996-3008.
5
Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels.通过SDS-聚丙烯酰胺凝胶电泳对多肽链进行分子量估计
Biochem Biophys Res Commun. 1967 Sep 7;28(5):815-20. doi: 10.1016/0006-291x(67)90391-9.
6
7
Lac repressor binding to synthetic DNAs of defined nucleotide sequence.乳糖阻遏蛋白与特定核苷酸序列的合成DNA的结合。
Proc Natl Acad Sci U S A. 1972 Mar;69(3):761-4. doi: 10.1073/pnas.69.3.761.
8
Evidence for two sites in the lac promoter region.乳糖操纵子启动子区域中两个位点的证据。
J Mol Biol. 1972 Aug 14;69(1):155-60. doi: 10.1016/0022-2836(72)90031-9.
9
The binding of cyclic adenosine monophosphate receptor to deoxyribonucleic acid.
J Biol Chem. 1972 Jul 10;247(13):4264-9.
Zotero 插件