Kawai K, Eguchi Y
J Biochem. 1980 Nov;88(5):1227-31. doi: 10.1093/oxfordjournals.jbchem.a133090.
Blue dextran or Cibacron Blue F3GA has been shown to inhibit yeast phosphoglycerate kinase [EC 2.7.2.3] competitively with respect to ATP (Thompson et al. (1975) Proc. Natl. Acad. Sci. U.S. 72, 663--667; Beissner and Rudolph (1979) J. Biol. Chem. 254, 6273--6277). However, we have found that phosphoglycerate kinase of Lactobacillus plantarum was inhibited by Cibacron Blue F3GA, the blue chromophore of blue dextran, noncompetitively with respect to ATP, but competitively with respect to 3-phosphoglycerate. Further inhibition studies with Cibacron Blue F3GA suggest that one molecule of the dye was bound per molecule of phosphoglycerate kinase at a saturated level of either substrate, but two molecules of the dye were bound per molecule of the kinase with an unsaturated level of either substrate used as a fixed substrate. Furthermore, phosphoglycerate mutase [EC 2.7.5.3] of Leuconostoc dextranicum was also inhibited by Cibacron Blue F3GA competitively with respect to 3-phosphoglycerate and noncompetitively with respect to 2,3-bisphosphoglycerate. These results suggest that the 3-phosphoglycerate-binding site on both phosphoglycerate kinase and phosphoglycerate mutase can interact with Cibacron Blue F3GA.
蓝色葡聚糖或Cibacron Blue F3GA已被证明能相对于ATP竞争性抑制酵母磷酸甘油酸激酶EC 2.7.2.3《美国国家科学院院刊》72, 663 - 667；Beissner和Rudolph，(1979)《生物化学杂志》254, 6273 - 6277)。然而，我们发现植物乳杆菌的磷酸甘油酸激酶被蓝色葡聚糖的蓝色发色团Cibacron Blue F3GA抑制，相对于ATP是非竞争性的，但相对于3 - 磷酸甘油酸是竞争性的。用Cibacron Blue F3GA进行的进一步抑制研究表明，在任一底物饱和水平下，每分子磷酸甘油酸激酶结合一分子染料，但在任一底物不饱和水平用作固定底物时，每分子激酶结合两分子染料。此外，右旋糖明串珠菌的磷酸甘油酸变位酶[EC 2.7.5.3]也被Cibacron Blue F3GA抑制，相对于3 - 磷酸甘油酸是竞争性的，相对于2,3 - 二磷酸甘油酸是非竞争性的。这些结果表明，磷酸甘油酸激酶和磷酸甘油酸变位酶上的3 - 磷酸甘油酸结合位点都能与Cibacron Blue F3GA相互作用。
