Chabi Malika, Goulas Estelle, Leclercq Celine C, de Waele Isabelle, Rihouey Christophe, Cenci Ugo, Day Arnaud, Blervacq Anne-Sophie, Neutelings Godfrey, Duponchel Ludovic, Lerouge Patrice, Hausman Jean-François, Renaut Jenny, Hawkins Simon
From the ‡Université Lille, CNRS, UMR 8576, UGSF - Unité de Glycobiologie Structurale et Fonctionnelle, F 59000 Lille, France.
§Department Environmental Research and Innovation (ERIN), Luxembourg Institute of Science and Technology (LIST), L-4422 Belvaux, Luxembourg.
Mol Cell Proteomics. 2017 Sep;16(9):1634-1651. doi: 10.1074/mcp.M116.063727. Epub 2017 Jul 13.
Experimentally-generated (nanoLC-MS/MS) proteomic analyses of four different flax organs/tissues (inner-stem, outer-stem, leaves and roots) enriched in proteins from 3 different sub-compartments (soluble-, membrane-, and cell wall-proteins) was combined with publically available data on flax seed and whole-stem proteins to generate a flax protein database containing 2996 nonredundant total proteins. Subsequent multiple analyses (MapMan, CAZy, WallProtDB and expert curation) of this database were then used to identify a flax cell wall proteome consisting of 456 nonredundant proteins localized in the cell wall and/or associated with cell wall biosynthesis, remodeling and other cell wall related processes. Examination of the proteins present in different flax organs/tissues provided a detailed overview of cell wall metabolism and highlighted the importance of hemicellulose and pectin remodeling in stem tissues. Phylogenetic analyses of proteins in the cell wall proteome revealed an important paralogy in the class IIIA xyloglucan endo-transglycosylase/hydrolase (XTH) family associated with xyloglucan endo-hydrolase activity.Immunolocalisation, FT-IR microspectroscopy, and enzymatic fingerprinting indicated that flax fiber primary/S1 cell walls contained xyloglucans with typical substituted side chains as well as glucuronoxylans in much lower quantities. These results suggest a likely central role of xyloglucans and endotransglucosylase/hydrolase activity in flax fiber formation and cell wall remodeling processes.
对富含来自3个不同亚组分（可溶性蛋白、膜蛋白和细胞壁蛋白）蛋白质的4种不同亚麻器官/组织（茎内部、茎外部、叶片和根）进行实验性生成的（纳升级液相色谱-串联质谱法）蛋白质组分析，并结合亚麻籽和全茎蛋白的公开可用数据，生成了一个包含2996个非冗余总蛋白的亚麻蛋白数据库。随后对该数据库进行了多次分析（MapMan、CAZy、WallProtDB和专家整理），以鉴定出一个由456个非冗余蛋白组成的亚麻细胞壁蛋白质组，这些蛋白定位于细胞壁和/或与细胞壁生物合成、重塑及其他细胞壁相关过程有关。对不同亚麻器官/组织中存在的蛋白质进行检查，提供了细胞壁代谢的详细概述，并突出了半纤维素和果胶重塑在茎组织中的重要性。对细胞壁蛋白质组中的蛋白质进行系统发育分析，揭示了与木葡聚糖内切水解酶活性相关的IIIA类木葡聚糖内切转糖基酶/水解酶（XTH）家族中的一个重要旁系同源现象。免疫定位、傅里叶变换红外光谱显微镜和酶指纹分析表明，亚麻纤维初生/S1细胞壁含有具有典型取代侧链的木葡聚糖以及数量少得多的葡糖醛酸木聚糖。这些结果表明，木葡聚糖和内转葡糖基酶/水解酶活性在亚麻纤维形成和细胞壁重塑过程中可能起着核心作用。
