Gábrišová Daša, Klubicová Katarína, Danchenko Maksym, Gömöry Dušan, Berezhna Valentyna V, Skultety Ludovit, Miernyk Ján A, Rashydov Namik, Hajduch Martin
Department of Developmental and Reproduction Biology, Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences Nitra, Slovakia.
Institute of Virology, Slovak Academy of SciencesBratislava, Slovakia; Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of UkraineKyiv, Ukraine.
Front Plant Sci. 2016 Jan 13;6:1215. doi: 10.3389/fpls.2015.01215. eCollection 2015.
Plants continue to flourish around the site of the Chernobyl Nuclear Power Plant disaster. The ability of plants to transcend the radio-contaminated environment was not anticipated and is not well understood. The aim of this study was to evaluate the proteome of flax (Linum usitatissimum L.) during seed filling by plants grown for a third generation near Chernobyl. For this purpose, seeds were harvested at 2, 4, and 6 weeks after flowering and at maturity, from plants grown in either non-radioactive or radio-contaminated experimental fields. Total proteins were extracted and the two-dimensional gel electrophoresis (2-DE) patterns analyzed. This approach established paired abundance profiles for 130 2-DE spots, e.g., profiles for the same spot across seed filling in non-radioactive and radio-contaminated experimental fields. Based on Analysis of Variance (ANOVA) followed by sequential Bonferroni correction, eight of the paired abundance profiles were discordant. Results from tandem mass spectrometry show that four 2-DE spots are discordant because they contain fragments of the cupin superfamily-proteins. Most of the fragments were derived from the N-terminal half of native cupins. Revisiting previously published data, it was found that cupin-fragments were also involved with discordance in paired abundance profiles of second generation flax seeds. Based on these observations we present an updated working model for the growth and reproductive success of flax in a radio-contaminated Chernobyl environment. This model suggests that the increased abundance of cupin fragments or isoforms and monomers contributes to the successful growth and reproduction of flax in a radio-contaminated environment.
在切尔诺贝利核电站灾难发生地周围，植物依然繁茂生长。植物超越受放射性污染环境的能力出乎人们的意料，且尚未得到充分理解。本研究的目的是评估在切尔诺贝利附近种植的第三代亚麻（Linum usitatissimum L.）在种子灌浆期的蛋白质组。为此，从生长在非放射性或受放射性污染试验田中的植株上，于开花后2周、4周、6周以及成熟期收获种子。提取总蛋白并分析二维凝胶电泳（2-DE）图谱。这种方法为130个2-DE斑点建立了配对丰度图谱，例如，在非放射性和受放射性污染试验田中，同一点在种子灌浆期的图谱。基于方差分析（ANOVA）并随后进行连续Bonferroni校正，8个配对丰度图谱不一致。串联质谱结果表明，4个2-DE斑点不一致是因为它们包含了cupin超家族蛋白的片段。大多数片段源自天然cupin的N端半段。回顾先前发表的数据发现，cupin片段也与第二代亚麻种子配对丰度图谱的不一致有关。基于这些观察结果，我们提出了一个更新的工作模型，用于解释亚麻在受放射性污染的切尔诺贝利环境中的生长和繁殖成功情况。该模型表明，cupin片段、异构体和单体丰度的增加有助于亚麻在受放射性污染的环境中成功生长和繁殖。
