Almeida Junior Luiz Domingues, Quaglio Ana Elisa Valencise, de Almeida Costa Celso Acácio Rodrigues, Di Stasi Luiz Claudio
Luiz Domingues Almeida Junior, Ana Elisa Valencise Quaglio, Celso Acácio Rodrigues de Almeida Costa, Luiz Claudio Di Stasi, Laboratory of Phytomedicines, Pharmacology and Biotechnology (PhytoPharmaTech), Department of Pharmacology, Institute of Biosciences, Universidade Estadual Paulista UNESP, Botucatu, São Paulo, CEP 18618-970, Brazil.
World J Gastroenterol. 2017 Jun 28;23(24):4369-4380. doi: 10.3748/wjg.v23.i24.4369.
To investigate the effects of Ground Cherry ( L.) standardized supercritical CO extract in trinitrobenzenesulphonic acid (TNBS) model of rat intestinal inflammation.
The animals were divided into groups that received vehicle or extract (PACO) orally at the doses 25, 50 and 100 mg/kg daily by 5 d before TNBS damage. Protective effects of PACO were assessed by macroscopic analysis, biochemical determinations of the levels of myeloperoxidase (MPO), alkaline phosphatase (ALP), glutathione and cytokines (such as INF-γ, IL-1β, IL-6, IL-10 and TNF-α), gene expression evaluation (including Hsp70, heparanase, NF-κB, mitogen-activated protein kinases (Mapk) 1, 3, 6 and 9, and the mucins genes Muc 1, 2, 3 and 4) and histopathological studies using optical, and electronic (transmission and scanning) microscopy.
PACO extract promoted a significant reduction in MPO and ALP activities, reducing oxidative stress and neutrophil infiltration. These effects were accompanied by significant reduction of colonic levels of IFN-γ and IL-6 and down-regulation of heparanase, Hsp70, Mapk3, Mapk9, Muc1 and Muc2 genes expression when compared with TNBS-control animals. In addition, protective effects were also evidenced by reduced neutrophil infiltration, recovery of cell architecture and replacement of mucin by histopathological and ultrastructural analysis.
supercritical CO extract is an intestinal anti-inflammatory product that modulates oxidative stress, immune response and expression of inflammatory mediators, with potentially utility for treating inflammatory bowel disease.
研究酸浆标准化超临界CO₂提取物对三硝基苯磺酸(TNBS)诱导的大鼠肠道炎症模型的影响。
将动物分为几组,在TNBS损伤前5天,每天口服给予溶剂或提取物(PACO),剂量分别为25、50和100mg/kg。通过宏观分析、髓过氧化物酶(MPO)、碱性磷酸酶(ALP)、谷胱甘肽和细胞因子(如INF-γ、IL-1β、IL-6、IL-10和TNF-α)水平的生化测定、基因表达评估(包括Hsp70、乙酰肝素酶、NF-κB、丝裂原活化蛋白激酶(Mapk)1、3、6和9,以及粘蛋白基因Muc 1、2、3和4)以及使用光学显微镜和电子显微镜(透射和扫描)进行组织病理学研究来评估PACO的保护作用。
与TNBS对照组动物相比,PACO提取物显著降低了MPO和ALP活性,减轻了氧化应激和中性粒细胞浸润。这些作用伴随着结肠中IFN-γ和IL-6水平的显著降低以及乙酰肝素酶、Hsp70、Mapk3、Mapk9、Muc1和Muc2基因表达的下调。此外,组织病理学和超微结构分析显示中性粒细胞浸润减少、细胞结构恢复以及粘蛋白替代,也证明了其保护作用。
超临界CO₂提取物是一种肠道抗炎产品,可调节氧化应激、免疫反应和炎症介质的表达,对治疗炎症性肠病具有潜在的应用价值。