Zhu Ning, Cai Changhong, Zhou Aiming, Zhao Xuyong, Xiang Yijia, Zeng Chunlai
Department of Cardiology, The Fifth Affiliated Hospital of Wenzhou Medical University, Lishui Central Hospital, Zhejiang Province 323000, China.
Department of Cardiology, The Third Clinical College of Wenzhou Medical University, Wenzhou People's Hospital, Wenzhou, Zhejiang Province 325000, China.
Biomed Res Int. 2017;2017:4237973. doi: 10.1155/2017/4237973. Epub 2017 Jun 19.
Schisandrin B (ScB), isolated from (), is a traditional Chinese medicine with proven cardioprotective and neuroprotective effects. However, it is unclear whether ScB also has beneficial effects on rat hind limb ischemia/reperfusion (I/R) injury model. In this study, ScB (20 mg/kg, 40 mg/kg, and 80 mg/kg) was administered via oral gavage once daily for 5 days before the surgery. After 6 h ischemia and 24 h reperfusion of left hind limb, ScB reduced I/R induced histological changes and edema. ScB also suppressed the oxidative stress through decreasing MDA level and increasing SOD activity. Moreover, above changes were associated with downregulated TNF- mRNA expression and reduced level of IL-1β in plasma. Meanwhile, ScB treatment downregulated activation of p38MAPK, ERK1/2, and NF-B in ischemic skeletal muscle. These results demonstrate that ScB treatment could prevent hind limb I/R skeletal muscle injury possibly by attenuating oxidative stress and inflammation via p38MAPK, ERK1/2, and NF-B pathways.
五味子乙素（ScB）是从（此处原文缺失相关植物名称）中分离得到的一种传统中药，已证实具有心脏保护和神经保护作用。然而，ScB对大鼠后肢缺血/再灌注（I/R）损伤模型是否也有有益作用尚不清楚。在本研究中，于手术前5天每天经口灌胃给予ScB（20毫克/千克、40毫克/千克和80毫克/千克），连续给药5天。左后肢缺血6小时和再灌注24小时后，ScB减轻了I/R诱导的组织学变化和水肿。ScB还通过降低丙二醛（MDA）水平和增加超氧化物歧化酶（SOD）活性来抑制氧化应激。此外，上述变化与肿瘤坏死因子（TNF）-mRNA表达下调以及血浆中白细胞介素-1β水平降低有关。同时，ScB处理下调了缺血骨骼肌中p38丝裂原活化蛋白激酶（p38MAPK）、细胞外信号调节激酶1/2（ERK1/2）和核因子κB（NF-κB）的激活。这些结果表明，ScB处理可能通过p38MAPK、ERK1/2和NF-κB途径减轻氧化应激和炎症，从而预防后肢I/R骨骼肌损伤。
