Qin Benlin, Liu Xuecong, Cui Haiming, Ma Yue, Wang Zimin, Han Jing
a School of Pharmaceutical Engineering , Shenyang Pharmaceutical University , Shenyang , Liaoning , P. R. China.
Prep Biochem Biotechnol. 2017 Oct 21;47(9):881-888. doi: 10.1080/10826068.2017.1350980. Epub 2017 Jul 14.
In this study, an efficient ultrasound-assisted aqueous two-phase extraction method was used for the extraction of anthocyanins from Lycium ruthenicum Murr. An ethanol/ammonium sulfate system was chosen for the aqueous two-phase system due to its fine partitioning and recycling behaviors. Single-factor experiments were conducted to determine the optimized composition of the system, and the response surface methodology was used for the further optimization of the ultrasound-assisted aqueous two-phase extraction. The optimal conditions were as follows: a salt concentration of 20%, an ethanol concentration of 25%, an extraction time of 33.7 min, an extraction temperature of 25°C, a liquid/solid ratio of 50:1 w/w, pH value of 3.98, and an ultrasound power of 600 W. Under the above conditions, the yields of anthocyanins reached 4.71 mg/g dry sample. For the further purification, D-101 resin was used, and the purity of anthocyanins reached 25.3%. In conclusion, ultrasound-assisted aqueous two-phase extraction was an efficient, ecofriendly, and economical method, and it may be a promising technique for extracting bioactive components from plants.
在本研究中，采用一种高效的超声辅助双水相萃取法从黑果枸杞中提取花色苷。由于乙醇/硫酸铵体系具有良好的分配和循环性能，因此选择该体系作为双水相体系。通过单因素实验确定体系的优化组成，并采用响应面法对超声辅助双水相萃取进行进一步优化。最佳条件如下：盐浓度20%、乙醇浓度25%、萃取时间33.7分钟、萃取温度25℃、液固比50:1 w/w、pH值3.98、超声功率600W。在上述条件下，花色苷得率达到4.71mg/g干样。为进一步纯化，使用D-101树脂，花色苷纯度达到25.3%。总之，超声辅助双水相萃取是一种高效、环保且经济的方法，可能是从植物中提取生物活性成分的一种有前景的技术。
