Gao Yuan, Ji Yubin, Wang Fuling, Li Wenlan, Zhang Xiaomeng, Niu Zhihui, Wang Zhuo
School of Pharmacy, Harbin University of Commerce, Harbin, Heilongjiang 150076 People's Republic of China.
Post-Doctoral Research Center of Traditional Chinese Medicine, Harbin University of Commerce, Harbin, Heilongjiang 150076 People's Republic of China.
Food Sci Biotechnol. 2021 Nov 26;30(13):1709-1719. doi: 10.1007/s10068-021-00994-w. eCollection 2021 Dec.
Blueberry residue is usually discarded as waste, but has a high anthocyanins content. The extraction method of anthocyanins from blueberry residue with ultrasonic assisted dual-aqueous phase system was optimized. In terms of the principle of central group and design (CCD) experimental design, three-factor and five-level response surface analysis was adopted to optimize the extraction conditions with the extraction rate of anthocyanins. The optimum extraction rate of anthocyanin was 12.372 ± 0.078 mg/g. Anthocyanin extract could protect the pBR322 DNA oxidative damage induced by Fenton reagent, increase the superoxide dismutase(SOD) and glutathione peroxidase (GSH-Px) enzyme activities, and decrease the HO-induced cell apoptosis of human normal liver cell (LO2 cell). The study indicates that the extraction rate of anthocyanin was increased by optimized ultrasonic assisted dual-aqueous phase system. The anthocyanin extract could protect DNA and LO2 cell from oxidative damage.
蓝莓残渣通常作为废弃物被丢弃，但它含有高含量的花青素。对采用超声辅助双水相体系从蓝莓残渣中提取花青素的方法进行了优化。依据中心组合设计（CCD）实验设计原理，采用三因素五水平响应面分析，以花青素提取率为指标对提取条件进行优化。花青素的最佳提取率为12.372±0.078毫克/克。花青素提取物可保护pBR322 DNA免受芬顿试剂诱导的氧化损伤，提高超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）的酶活性，并减少HO诱导的人正常肝细胞（LO2细胞）凋亡。研究表明，优化后的超声辅助双水相体系提高了花青素的提取率。花青素提取物可保护DNA和LO2细胞免受氧化损伤。
