Ginther O J, Domingues R R, Siddiqui M A R, Dangudubiyyam S V
Eutheria Foundation, Cross Plains, WI 53528, USA; Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI 53706, USA.
Eutheria Foundation, Cross Plains, WI 53528, USA; Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI 53706, USA.
Theriogenology. 2017 Sep 15;100:42-49. doi: 10.1016/j.theriogenology.2017.05.028. Epub 2017 May 31.
Diameter deviation is the beginning of a decrease in growth rate of the largest subordinate follicle (SF) and a continuing growth rate of the dominant follicle (DF). In wave 1 in cattle, deviation begins 2 or 3 days after ovulation when the future DF is about 8.5 mm. Gray scale and power-flow Doppler ultrasound examinations were done in experiment 1 (daily examinations, n = 13) and experiment 2 (examinations every 8 h, n = 15). Blood flow and an anechoic layer in the follicle wall were normalized to the beginning of diameter deviation (day 0 or hour 0). Only waves with conventional diameter deviation (68% of waves) were used as identified by: (1) future SF greater than 7.0 mm when DF was 8.5 mm and (2) future DF and SF did not switch in diameter rank. In experiment 1, deviations in the extent of blood-flow signals and in the extent of anechoic layer began on the same day as deviation in diameter. In experiment 2, deviations in diameter, surface area (πd), and anechoic layer began in synchrony, and deviation in blood-flow signals began 16 h earlier. Blood-flow deviation before diameter deviation was shown by (1) a first difference (P < 0.02) between follicles at hour -16 and (2) development during the hours -24 to 0 of a greater (P < 0.05) percentage difference between follicles in blood-flow signals (11.1 ± 2.3%) than in surface area (7.4 ± 0.7%) or diameter (4.5 ± 0.4%). Results supported the hypothesis that the extent of blood flow in the future dominant and subordinate follicles deviates before diameter deviates. A similar hypothesis for anechoic layer was not supported; diameter and anechoic layer deviated in synchrony.
直径偏差是最大次级卵泡（SF）生长速率下降以及优势卵泡（DF）持续生长速率的开始。在牛的第1个卵泡波中，排卵后2或3天，当未来的DF约为8.5毫米时开始出现偏差。在实验1（每日检查，n = 13）和实验2（每8小时检查，n = 15）中进行了灰阶和能量多普勒超声检查。卵泡壁中的血流和无回声层以直径偏差开始时（第0天或第0小时）为基准进行标准化。只有具有传统直径偏差的波（占波的68%）被用作分析对象，其判定标准为：（1）当DF为8.5毫米时，未来的SF大于7.0毫米；（2）未来的DF和SF在直径排名上没有互换。在实验1中，血流信号范围和无回声层范围的偏差与直径偏差在同一天开始。在实验2中，直径、表面积（πd）和无回声层的偏差同步开始，而血流信号的偏差提前16小时开始。直径偏差前的血流偏差表现为：（1）在-16小时时卵泡之间的一阶差分（P < 0.02）；（2）在-24至0小时期间，卵泡之间血流信号的百分比差异（11.1 ± 2.3%）大于表面积（7.4 ± 0.7%）或直径（4.5 ± 0.4%）（P < 0.05）。结果支持了以下假设：未来优势卵泡和次级卵泡中的血流范围在直径偏差之前就出现了偏差。关于无回声层的类似假设未得到支持；直径和无回声层同步出现偏差。
