Department of Obstetrics & Gynecology and Reproduction, Faculty of Veterinary Medicine, Kafkas University, Kars, Turkey.
Anim Reprod Sci. 2011 Feb;123(3-4):149-56. doi: 10.1016/j.anireprosci.2011.01.001. Epub 2011 Jan 14.
Two experiments were conducted to test the hypothesis that there are dynamic changes in follicular blood flow during follicular deviation and that nitric oxide (NO) in follicular fluid (FF) plays a role in regulation of follicular blood flow. In Experiment I, follicular blood flow of the two largest follicles was monitored by using Power Doppler ultrasonography during follicular deviation in sixteen follicular waves during eight estrous cycles in eight cows. Blood flow did not differ (P>0.05) between the dominant follicle (DF) and the largest subordinate follicle (SF) until the beginning of the deviation of the follicular size, but was higher (P<0.05) in DF than in the largest SF one and two days after the beginning of diameter deviation in ovulatory (n=5) and atretic (n=11) waves; respectively. In Experiment II, FF was aspirated from DF and the largest SF on the day of diameter deviation (DF, n=6; SF, n=6) and two days later (DF, n=12; SF, n=9). Nitric oxide did not differ (P>0.05) between DF and the largest SF on the day of diameter deviation but, one or two days after observed diameter deviation NO concentrations were lower (P<0.01) in DF compared to the largest SF. On the day of diameter deviation and two days later E2 levels in FF were higher (P<0.01) in DF than in the largest SF. P4 concentrations in FF were higher (P<0.05) in DF than in the largest SF on the day of diameter deviation, but did not (P>0.05) differ two days later. E2/P4 ratio in FF was the same (P>0.05) in DF and the largest SF on the day of diameter deviation, but was higher (P<0.01) in DF than in the largest SF one or two days later. In conclusion, area of follicular blood flow of DF and the largest SF increased in parallel with follicular size during follicular deviation. Furthermore, there were relationships between changes in follicular blood flow, NO concentrations and E2/P4 ratio in FF following the beginning of diameter deviation in cattle.
进行了两项实验来检验以下假说，即在卵泡偏离过程中卵泡内血流存在动态变化，并且卵泡液（FF）中的一氧化氮（NO）在调节卵泡内血流中发挥作用。在实验 I 中，在 8 头奶牛的 8 个发情周期的 16 个卵泡波中，使用能量多普勒超声监测两个最大卵泡的卵泡内血流。在卵泡大小开始偏离之前，主导卵泡（DF）和最大的从属卵泡（SF）之间的血流没有差异（P>0.05），但在开始偏离直径后两天，DF 的血流高于（P<0.05）DF 和最大的 SF 在排卵（n=5）和退化（n=11）波中；分别。在实验 II 中，在直径偏离的当天（DF，n=6；SF，n=6）和两天后（DF，n=12；SF，n=9）从 DF 和最大的 SF 中吸出 FF。在直径偏离的当天，DF 和最大的 SF 之间的 NO 没有差异（P>0.05），但在观察到直径偏离后 1 或 2 天，DF 中的 NO 浓度比最大的 SF 低（P<0.01）。在直径偏离的当天和两天后，DF 中的 FF 中的 E2 水平高于（P<0.01）最大的 SF。在直径偏离的当天，DF 中的 FF 中的 P4 浓度高于（P<0.05）最大的 SF，但两天后没有差异（P>0.05）。在直径偏离的当天，DF 和最大的 SF 中的 FF 中的 E2/P4 比率相同（P>0.05），但在直径偏离后 1 或 2 天，DF 中的 E2/P4 比率高于最大的 SF（P<0.01）。总之，在卵泡偏离过程中，DF 和最大的 SF 的卵泡内血流面积与卵泡大小呈平行增加。此外，在牛的卵泡直径开始偏离后，FF 中的卵泡内血流变化、NO 浓度和 E2/P4 比率之间存在关系。
