Metabolic Engineering Research Laboratory, Science and Engineering Institutes, Agency for Science, Technology and Research, Singapore.
Metabolic Engineering Research Laboratory, Science and Engineering Institutes, Agency for Science, Technology and Research, Singapore; Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, United States.
Bioorg Med Chem. 2018 Apr 1;26(7):1275-1284. doi: 10.1016/j.bmc.2017.06.043. Epub 2017 Jun 28.
Biocatalysis has been increasingly used for pharmaceutical synthesis in an effort to make manufacturing processes greener and more sustainable. Biocatalysts that possess excellent activity, specificity, thermostability and solvent-tolerance are highly sought after to meet the requirements of practical applications. Generating biocatalysts with these specific properties can be achieved by either discovery of novel biocatalysts or protein engineering. Meanwhile, chemoenzymatic routes have also been designed and developed for pharmaceutical synthesis on an industrial scale. This review discusses the recent discoveries, engineering, and applications of biocatalysts for the synthesis of pharmaceuticals and pharmaceutical intermediates. Key classes of biocatalysts include reductases, oxidases, hydrolases, lyases, isomerases, and transaminases.
生物催化在药物合成中的应用日益增多，旨在使制造工艺更加环保和可持续。为了满足实际应用的要求，人们迫切需要具有出色的活性、特异性、热稳定性和溶剂耐受性的生物催化剂。通过发现新型生物催化剂或蛋白质工程，可以获得具有这些特定性质的生物催化剂。同时，也已经设计和开发了用于药物合成的化学酶法路线。本文综述了用于药物和药物中间体合成的生物催化剂的最新发现、工程和应用。关键的生物催化剂包括还原酶、氧化酶、水解酶、裂解酶、异构酶和转氨酶。
