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利用产生热稳定α-淀粉酶和纤维素酶的微生物培养物在不同 pH 值和温度下生物降解食物垃圾。

Biodegradation of food waste using microbial cultures producing thermostable α-amylase and cellulase under different pH and temperature.

机构信息

College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi, China; Department of Biotechnology, Amicable Knowledge Solution University, Satna, Madhya Pradesh, India.

Sino-Forest Applied Research Centre for Pearl River Delta Environment and Department of Biology, Hong Kong Baptist University, Hong Kong, China.

出版信息

Bioresour Technol. 2018 Jan;248(Pt B):160-170. doi: 10.1016/j.biortech.2017.06.160. Epub 2017 Jun 30.

Abstract

The aim of this work was to study the biodegradation of food waste employing thermostable α-amylase and cellulase enzymes producing bacteria. Four potential isolates were identified which were capable of producing maximum amylase and cellulase and belong to the amylolytic strains, Brevibacillus borstelensis and Bacillus licheniformis; cellulolytic strains, Bacillus thuringiensis and Bacillus licheniformis, respectively. These strains were selected based on its higher cell density, enzymatic activities and stability at a wide range of pH and temperature compared to other strains. The results indicated that 1:1 ratio of pre and post consumed food wastes (FWs) were helpful to facilitate the degradation employing bacterial consortium. In addition, organic matter decomposition and chemical parameters of the end product quality also indicated that bacterial consortium was very effective for 1:1 ratio of FWs degradation as compared to the other treatments.

摘要

本工作旨在研究利用产热稳定α-淀粉酶和纤维素酶的细菌对食物垃圾进行生物降解。鉴定出了四个有潜力的分离菌,它们能够产生最大量的淀粉酶和纤维素酶,分别属于解淀粉芽孢杆菌和地衣芽孢杆菌(amylolytic strains);苏云金芽孢杆菌和地衣芽孢杆菌(cellulolytic strains)。与其他菌株相比,这些菌株的细胞密度更高,酶活性和在较宽的 pH 和温度范围内的稳定性更高,因此被选中。结果表明,采用细菌混合菌对 1:1 比例的预消耗和消耗后的食物垃圾(FWs)进行降解是有帮助的。此外,有机物质分解和终产物质量的化学参数也表明,与其他处理相比,细菌混合菌对 1:1 比例的 FWs 降解非常有效。

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