Hefei National Laboratory for Physical Sciences at the Microscale, Department of Chemical Physics, University of Science and Technology of China, Hefei, Anhui, China.
Synergetic Innovation Center of Quantum Information & Quantum Physics, University of Science and Technology of China, Hefei, Anhui, China.
Photochem Photobiol. 2018 Jan;94(1):61-68. doi: 10.1111/php.12808. Epub 2017 Sep 8.
The photochemical reaction between 1,2-naphthoquinone (NQ) and adenine was investigated using nanosecond time-resolved laser flash photolysis. With photolysis at 355 nm, the lowest triplet state T of NQ was produced via intersystem crossing from its singlet excited state. The triplet-triplet absorption of the state contributes three bands of transient spectra at 374, 596 and 650 nm, respectively, in pure acetonitrile and binary water-acetonitrile solutions. In the presence of adenine, the observation of A· (at 363 nm) and NQ+H· radical (at 343 and 485 nm) indicates a multistep mechanism of electron transfer process followed by a proton transfer between NQ* and adenine. By fitting with the Stern-Volmer relationship, the quenching rate constant k of NQ* by adenine in binary water-acetonitrile solutions (4/1, volume ratio, v/v) is determined as 1.66 × 10 m s . Additionally, no spectral evidence confirms the existence of electron transfer between NQ* with thymine, cytosine and uracil.
使用纳秒时间分辨激光闪光光解技术研究了 1,2-萘醌（NQ）与腺嘌呤之间的光化学反应。在 355nm 的光解下，NQ 的最低三重态 T 通过系间窜越从其单重激发态产生。该状态的三重态-三重态吸收在纯乙腈和二元水-乙腈溶液中分别贡献三个瞬态光谱带，分别在 374、596 和 650nm 处。在腺嘌呤存在下，观察到 A·（在 363nm 处）和 NQ+H·自由基（在 343 和 485nm 处）表明电子转移过程后是 NQ和腺嘌呤之间的质子转移的多步骤机制。通过与 Stern-Volmer 关系拟合，确定了在二元水-乙腈溶液（4/1，体积比，v/v）中 NQ被腺嘌呤猝灭的速率常数 k 为 1.66×10 m s 。此外，没有光谱证据证实 NQ*与胸腺嘧啶、胞嘧啶和尿嘧啶之间存在电子转移。
