Mehta K, Claringbold P, Lopez-Berestein G
J Immunol. 1986 Jun 1;136(11):4206-12.
Culture of mouse resident peritoneal macrophages (PM) in serum-containing medium causes a rapid and marked induction of the enzyme tissue transglutaminase (tissue TGase). Coculture of PM with amphotericin B (AmpB) inhibited the serum-induced expression and accumulation of tissue TGase. The AmpB-mediated inhibition of tissue TGase was specific and was due to inhibition of enzyme synthesis. The serum-dependent induction of tissue TGase was inhibited in a dose-dependent fashion, and a complete inhibition was observed at 1.5 microgram/ml dose of AmpB. The inhibition was reversible; however, the time of recovery depended on the dose and time of exposure of the cells to AmpB. The present studies suggest that AmpB-mediated inhibition of tissue TGase is due to inhibition of the uptake of serum retinoids by PM.
在含血清培养基中培养小鼠常驻腹膜巨噬细胞（PM）会迅速且显著地诱导组织转谷氨酰胺酶（组织TGase）的产生。PM与两性霉素B（AmpB）共培养可抑制血清诱导的组织TGase的表达和积累。AmpB介导的对组织TGase的抑制具有特异性，且是由于对酶合成的抑制。血清依赖性诱导的组织TGase以剂量依赖性方式受到抑制，在1.5微克/毫升剂量的AmpB时观察到完全抑制。这种抑制是可逆的；然而，恢复时间取决于细胞暴露于AmpB的剂量和时间。目前的研究表明，AmpB介导的对组织TGase的抑制是由于PM对血清类视黄醇摄取的抑制。
