Dai Chaohui, Sun Li, Xia Riwei, Sun Shouyong, Zhu Guoqiang, Wu Shenglong, Bao Wenbin
Department of College of Animal Science and Technology, Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou, Jiangsu, People's Republic of China.
Department of College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, People's Republic of China.
3 Biotech. 2017 Aug;7(4):247. doi: 10.1007/s13205-017-0880-9. Epub 2017 Jul 15.
Alpha-(1,2)-fucosyltransferase (FUT1) gene has some influence on economically important traits and disease resistance. DNA methylation plays an important role in human diseases but is relatively poorly studied in pigs by regulating the mRNA expression of genes. The aim of this study was to analyze the influence of promoter methylation on the expression of FUT1 gene. We used bisulfite sequencing PCR (BSP) and qPCR to analyze the methylation of the FUT1 5'-flanking region and FUT1 mRNA expression in the duodenum of Sutai piglets from newborn to weaning. FUT1 contains three CpG islands upstream of the start codon, of which two are located in the putative promoter region containing multiple promoter elements and transcription factor binding sites, such as CpG islands, a CAAT box, SP1, and EARLY-SEQ 1. The CpG island between nucleotides -1762 and -580 had a low degree of methylation, and its methylation level was significantly lower in 35-day-old piglets than 8- and 18-day-old piglets (P < 0.05). FUT1 mRNA expression was significantly higher in 35-day-old piglets than 8- and 18-day-old piglets (P < 0.05). Pearson's correlation analysis showed that the methylation of the CpG island between nucleotides -1762 and -580 of FUT1 was significantly, negatively correlated with FUT1 mRNA expression (P < 0.05). These results demonstrate that differential methylation of CpG islands negatively regulates the expression of FUT1 in the porcine duodenum, suggesting a probable influence on the resistance of piglets to infection with ETEC F18.
α-(1,2)-岩藻糖基转移酶（FUT1）基因对经济重要性状和抗病性有一定影响。DNA甲基化在人类疾病中起重要作用，但在猪中通过调节基因的mRNA表达进行的研究相对较少。本研究旨在分析启动子甲基化对FUT1基因表达的影响。我们使用亚硫酸氢盐测序PCR（BSP）和qPCR分析了苏太仔猪从出生到断奶期间十二指肠中FUT1 5'-侧翼区域的甲基化和FUT1 mRNA表达。FUT1在起始密码子上游包含三个CpG岛，其中两个位于推定的启动子区域，该区域包含多个启动子元件和转录因子结合位点，如CpG岛、CAAT盒、SP1和EARLY-SEQ 1。核苷酸-1762至-580之间的CpG岛甲基化程度较低，其甲基化水平在35日龄仔猪中显著低于8日龄和18日龄仔猪（P < 0.
