Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.
Mol Biol Rep. 2012 Apr;39(4):4223-8. doi: 10.1007/s11033-011-1208-1. Epub 2011 Jul 19.
Alpha (1,2) fucosyltransferase (FUT1) gene has been identified as a candidate gene for controlling the expression of the receptor for ETEC F18. The genetic variations in the position of M307 nucleotide in open reading frame of FUT1 have been proposed as a marker for selecting ETEC F18 resistant pigs. The polymorphisms of M307 in FUT1 of breeding base group for ETEC F18 resistance of Sutai pigs (Duroc × Meishan) was detected and their correlations to some immune indexes, growth and development ability, carcass traits and meat quality were also analyzed, which aimed to investigate feasibility of further breeding for diseases resistance based on M307 of FUT1 for Sutai pigs. After digested by Hin6 I, M307 of FUT1 gene could be divided into three kinds of genotypes, AA, AG, and GG. The frequencies were 0.235, 0.609, and 0.156, respectively. The results indicated that Sutai pigs with the AA genotype in M307 of FUT1 gene not only have relatively strong general disease resistance ability in piglets, but also have higher growth and development ability and stable carcass traits and meat quality. It is entirely feasible to raise the new strains of Sutai pigs resistant to Escherichia coli F18 based on genetic marker of the M307 position in FUT1gene.
α(1,2)岩藻糖基转移酶(FUT1)基因已被确定为控制大肠杆菌 F18 受体表达的候选基因。FUT1 开放阅读框中 M307 核苷酸位置的遗传变异被提议作为选择大肠杆菌 F18 抗性猪的标记。检测了苏太猪(杜洛克×梅山)大肠杆菌 F18 抗性选育基础群 FUT1 中 M307 的多态性，并分析了其与一些免疫指标、生长发育能力、胴体性状和肉质的相关性，旨在探讨基于 FUT1 的 M307 进一步培育苏太猪疾病抗性的可行性。经 Hin6 I 消化后，FUT1 基因的 M307 可分为三种基因型，AA、AG 和 GG。频率分别为 0.235、0.609 和 0.156。结果表明，苏太猪 FUT1 基因 M307 位点的 AA 基因型不仅仔猪具有较强的一般抗病能力，而且具有较高的生长发育能力和稳定的胴体性状和肉质。基于 FUT1 基因 M307 位置的遗传标记，完全可以培育出抗大肠杆菌 F18 的苏太猪新品系。
