Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.
Genes (Basel). 2021 Oct 9;12(10):1586. doi: 10.3390/genes12101586.
Post-weaning diarrhea (PWD) is frequently associated with F18 infections in piglets. However, the underlying molecular mechanism concerning the resistance of F18 in local weaned piglets in China is not clearly understood. In the present study, by a comparative analysis of the transcriptome, a-1,3-fucosyltransferase () was evaluated as a key candidate correlated with resistance to F18 in Sutai and Meishan piglets. Functional analysis demonstrated that acts as a key positive regulator of F18 susceptibility in newly food accustomed piglets. However, the core promoter of was present at -500-(-206) bp (chr.2: g.73171117-g.73171616), comprising of 9 methylated CpG sites. Among these, the methylation levels of the two CpG sites (mC-3, mC-5) located in HIF1A and Sp1 transcription factor (TF) considerably associated with mRNA expression of ( < 0.05). Our findings indicated that the methylation of mC-3 and mC-5 sites has certain inhibitory effect on expression and promotes the resistance of F18 in piglets. The underlined study may explore as a new candidate target in F18 infection in Chinese local weaned piglets.
断奶后腹泻（PWD）常与仔猪 F18 感染有关。然而，中国本地断奶仔猪对 F18 抗性的潜在分子机制尚不清楚。在本研究中，通过对转录组的比较分析，发现 a-1,3-岩藻糖基转移酶（）是与 Sutai 和梅山仔猪对 F18 抗性相关的关键候选基因。功能分析表明，在新食物适应的仔猪中，作为 F18 易感性的关键正调控因子。然而，的核心启动子位于-500-(-206) bp（chr.2：g.73171117-g.73171616），包含 9 个甲基化 CpG 位点。其中，位于 HIF1A 和 Sp1 转录因子（TF）中的两个 CpG 位点（mC-3、mC-5）的甲基化水平与 mRNA 表达水平显著相关（<0.05）。我们的研究结果表明，mC-3 和 mC-5 位点的甲基化对的表达有一定的抑制作用，并促进了仔猪对 F18 的抗性。该研究可能为中国本地断奶仔猪 F18 感染提供了一个新的候选靶点。
