Starchenko Ivan I, Vitko Yulia N, Prylutskyi Oleksii K, Bilokon Sergii O, Vynnyk Nataliia I
Higher State Educational Establishment of Ukraine "Ukrainian Medical Stomatological Academy", Poltava, Ukraine.
Wiad Lek. 2017;70(3 pt 2):561-565.
Data related to the amount, size and morphological characteristics of cell elements of sensory ganglia at different stages of prenatal development has not been fully elucidated in recent scientific publications. At the same time publications considering the study of cell structure of trigeminal ganglion in the postnatal period confirm heterogeneity of its neurons. The aim of the research was to study morphological and immunohistochemical characteristics of human trigeminal ganglion neurons at 12-14 weeks of prenatal development.
The study was made on 24 trigeminal ganglions of 12 human fetuses at 12 to 14 weeks of prenatal development after abortion made on social and medical indications.
At the studied period of the intrauterine development nerve cells of the trigeminal ganglion significantly differed in size, tinctorial properties and degree of argentophility of the perikaryon. At the same time, the number of small nerve cells with an average diameter of less than 15 μm prevailed. Immunohistochemical study allowed detecting the apparent Bcl-2 expression in the overwhelming number of small neurons; the expression of this marker has been observed in 50% of cells of the medium-sized neurons. No Bcl-2 expression has been found in most of the large neurons. Almost all the neurons, regardless of the size, showed moderate Ki-67 expression, protein S-100. VEGF expression has also occurred in the vast majority of the nerve cells of all size groups.
近期科学出版物中尚未充分阐明产前发育不同阶段感觉神经节细胞成分的数量、大小和形态特征相关数据。同时，有关出生后三叉神经节细胞结构研究的出版物证实了其神经元的异质性。本研究的目的是研究产前发育12 - 14周时人三叉神经节神经元的形态和免疫组化特征。
对因社会和医学指征而流产的12例12至14周龄人类胎儿的24个三叉神经节进行了研究。
在宫内发育的研究阶段，三叉神经节的神经细胞在大小、染色特性和核周嗜银性程度上存在显著差异。同时，平均直径小于15μm的小神经细胞数量占优势。免疫组化研究发现绝大多数小神经元中有明显的Bcl - 2表达；在中等大小神经元的50%细胞中观察到该标志物的表达。在大多数大神经元中未发现Bcl - 2表达。几乎所有神经元，无论大小，均显示出中等程度的Ki - 67表达、蛋白S - 100表达。VEGF表达也出现在所有大小组的绝大多数神经细胞中。
