Cheng Ming-Fang, Lin Chun-Shu, Chen Yu-Hsin, Sung Ping-Jyun, Lin Shian-Ren, Tong Yi-Wen, Weng Ching-Feng
Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei 10086, Taiwan.
Division of Histology and Clinical Pathology, Hualian Army Forces General Hospital, Hualien 97144, Taiwan.
Mar Drugs. 2017 Jul 15;15(7):224. doi: 10.3390/md15070224.
Chemotherapy drugs for oral cancers always cause side effects and adverse effects. Currently natural sources and herbs are being searched for treated human oral squamous carcinoma cells (OSCC) in an effort to alleviate the causations of agents in oral cancers chemotherapy. This study investigates the effect of prodigiosin (PG), an alkaloid and natural red pigment as a secondary metabolite of , to inhibit human oral squamous carcinoma cell growth; thereby, developing a new drug for the treatment of oral cancer. In vitro cultured human OSCC models (OECM1 and SAS cell lines) were used to test the inhibitory growth of PG via cell cytotoxic effects (MTT assay), cell cycle analysis, and Western blotting. PG under various concentrations and time courses were shown to effectively cause cell death and cell-cycle arrest in OECM1 and SAS cells. Additionally, PG induced autophagic cell death in OECM1 and SAS cells by LC3-mediated P62/LC3-I/LC3-II pathway at the in vitro level. These findings elucidate the role of PG, which may target the autophagic cell death pathways as a potential agent in cancer therapeutics.
用于口腔癌的化疗药物总会引起副作用和不良反应。目前，人们正在寻找天然来源和草药来治疗人类口腔鳞状细胞癌（OSCC），以减轻口腔癌化疗中药物的副作用。本研究调查了灵菌红素（PG），一种作为次级代谢产物的生物碱和天然红色素，对抑制人类口腔鳞状癌细胞生长的作用；从而开发一种治疗口腔癌的新药。体外培养的人类OSCC模型（OECM1和SAS细胞系）用于通过细胞毒性作用（MTT法）、细胞周期分析和蛋白质印迹法测试PG的生长抑制作用。不同浓度和时间进程的PG显示能有效导致OECM1和SAS细胞死亡和细胞周期停滞。此外，在体外水平上，PG通过LC3介导的P62/LC3-I/LC3-II途径诱导OECM1和SAS细胞自噬性细胞死亡。这些发现阐明了PG的作用，它可能作为癌症治疗中的一种潜在药物靶向自噬性细胞死亡途径。
