Department of Drug and Food Control, Faculty of Pharmacy and Biotechnology Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Nat Prod Res. 2012;26(22):2078-83. doi: 10.1080/14786419.2011.622276. Epub 2011 Oct 10.
In this study, a red pigment of Serratia marcescens PTCC 1111 was purified and identified for antiproliferative activities in HT-29 and T47D cancer cell lines. (1)H-NMR spectroscopy and LC/MS analysis confirmed prodigiosin structure. The antiproliferative effects of prodigiosin were determined by employing the MTT assay. The changes in cell cycle pattern were studied with 4',6-diamidino-2-phenylindole (DAPI) reagent using flow cytometry assay, and Annexin V-PI method was used for apoptotic analysis. Results of MTT assay showed that HT-29 cells were more sensitive to prodigiosin than T47D cells. Prodigiosin-treated HT-29 cells showed increase in S phase and decrease in G2/M, but treated T47D cells showed cell cycle pattern relatively similar to Roswell Park Memorial Institute medium (RPMI). Apoptotic effect of prodigiosin was higher than doxorubicin in HT-29 cells. The data reported here indicate that prodigiosin is a promising antineoplastic agent that triggers apoptosis in different cancer cell lines.
在这项研究中，我们从粘质沙雷氏菌 PTCC 1111 中分离纯化了一种红色素，并对其在 HT-29 和 T47D 癌细胞系中的抗增殖活性进行了鉴定。（1）H-NMR 光谱和 LC/MS 分析证实了灵菌红素的结构。采用 MTT 法测定灵菌红素的抗增殖作用。通过使用 4'，6-二脒基-2-苯基吲哚（DAPI）试剂进行流式细胞术分析，研究细胞周期模式的变化，并用 Annexin V-PI 法进行凋亡分析。MTT 法的结果表明，HT-29 细胞对灵菌红素比 T47D 细胞更敏感。经灵菌红素处理的 HT-29 细胞 S 期增加，G2/M 期减少，但经处理的 T47D 细胞的细胞周期模式与罗威尔帕克纪念研究所培养基（RPMI）相对相似。灵菌红素在 HT-29 细胞中的凋亡作用强于阿霉素。这里报道的数据表明，灵菌红素是一种有前途的抗肿瘤药物，可在不同的癌细胞系中引发细胞凋亡。
