Heydarizadeh Parisa, Boureba Wafâa, Zahedi Morteza, Huang Bing, Moreau Brigitte, Lukomska Ewa, Couzinet-Mossion Aurélie, Wielgosz-Collin Gaëtane, Martin-Jézéquel Véronique, Bougaran Gaël, Marchand Justine, Schoefs Benoît
Metabolism, Bioengineering of Microalga Molecules and Applications (MIMMA), Mer Molécules Santé, UBL, IUML-FR 3473 CNRS, University of Le Mans, 72085 Le Mans, France.
Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan University of Technology, Isfahan 84156-83111, Iran.
Philos Trans R Soc Lond B Biol Sci. 2017 Sep 5;372(1728). doi: 10.1098/rstb.2016.0396.
In this study, we investigated the responses of cells acclimated to 300 µmol m s photon flux density to an increase (1000 µmol m s) or decrease (30 µmol m s) in photon flux densities. The light shift occurred abruptly after 5 days of growth and the acclimation to new conditions was followed during the next 6 days at the physiological and molecular levels. The molecular data reflect a rearrangement of carbon metabolism towards the production of phosphoenolpyruvic acid (PEP) and/or pyruvate. These intermediates were used differently by the cell as a function of the photon flux density: under low light, photosynthesis was depressed while respiration was increased. Under high light, lipids and proteins accumulated. Of great interest, under high light, the genes coding for the synthesis of aromatic amino acids and phenolic compounds were upregulated suggesting that the shikimate pathway was activated.This article is part of the themed issue 'The peculiar carbon metabolism in diatoms'.
在本研究中，我们调查了适应300 μmol m⁻² s⁻¹光子通量密度的细胞对光子通量密度增加（1000 μmol m⁻² s⁻¹）或降低（30 μmol m⁻² s⁻¹）的反应。光转移在生长5天后突然发生，随后在接下来的6天里，在生理和分子水平上跟踪细胞对新条件的适应情况。分子数据反映了碳代谢朝着磷酸烯醇丙酮酸（PEP）和/或丙酮酸生产的重新排列。这些中间产物被细胞根据光子通量密度以不同方式利用：在低光照下，光合作用受到抑制而呼吸作用增强。在高光照下，脂质和蛋白质积累。非常有趣的是；在高光照下，编码芳香族氨基酸和酚类化合物合成的基因被上调，这表明莽草酸途径被激活。本文是主题为“硅藻中独特的碳代谢”这一特刊的一部分。
