唾液酸在水疱性口炎病毒细胞受体中的作用。

Role of sialic acid in cell receptors for vesicular stomatitis virus.

作者信息

Superti F, Girmenta C, Seganti L, Orsi N

出版信息

Acta Virol. 1986 Jan;30(1):10-8.

Abstract

The role of sialic acid in the receptors for vesicular stomatitis virus (VSV) was investigated by treating chick embryo related (CER) cells and goose erythrocytes with neuraminidases from different sources and studying the effects produced on both infection and haemagglutination. The virus attachment to CER cells was reduced by their treatment with C. perfringens and A. ureafaciens neuraminidases and the susceptibility to viral infection of desialylated CER cells was recovered by coating with gangliosides immediately after enzymatic treatment. By contrast, enzymatic treatment of goose erythrocytes enhanced their agglutinability by VSV. The experiments carried out to test the ability of sialoglycolipids (gangliosides) and asialoglycolipids (cerebrosides) to inhibit virus attachment to CER cells or to goose erythrocytes showed that only gangliosides were able to inhibit VSV infection and haemagglutination.

摘要

通过用不同来源的神经氨酸酶处理鸡胚相关（CER）细胞和鹅红细胞，并研究其对感染和血凝产生的影响，来探究唾液酸在水泡性口炎病毒（VSV）受体中的作用。用产气荚膜梭菌和脲芽孢八叠球菌神经氨酸酶处理CER细胞后，病毒对CER细胞的附着减少，并且在酶处理后立即用神经节苷脂包被，去唾液酸化的CER细胞对病毒感染的敏感性得以恢复。相比之下，对鹅红细胞进行酶处理增强了它们被VSV凝集的能力。为测试唾液糖脂（神经节苷脂）和去唾液酸糖脂（脑苷脂）抑制病毒附着于CER细胞或鹅红细胞的能力而进行的实验表明，只有神经节苷脂能够抑制VSV感染和血凝。