Lundh B, Brockstedt U, Kristensson K
Department of Pathology (Neuropathology), Karolinska Institutet, Huddinge Hospital, Sweden.
Histochem J. 1989 Jan;21(1):33-43. doi: 10.1007/BF01002469.
Sections from the nasal cavity of 12-day-old Swiss albino mice (NMRI strain) were subjected to lectin histochemistry. A panel of biotinylated lectins (Con A, WGA, s-WGA, PNA, SBA, DBA and UEA I) and a horseradish peroxidase-conjugated lectin (GSA II) showed marked differences in binding to the respiratory and the neuroepithelial cells. SBA (affinity for galactose and N-acetylgalactosamine), PNA (galactose) and WGA (sialic acids and N-acetylglucosamine) labelled the receptor neurons in the olfactory and vomeronasal epithelium. DBA (N-acetylgalactosamine) labelled a subgroup of about 5% of the olfactory receptor neurons, but most neurons in the vomeronasal organ. UEA I (fucose) and s-WGA (N-acetylglucosamine) intensely labelled the entire nerve cell population in the vomeronasal organ, but in the olfactory epithelium the labelling with these lectins was stratified. In the respiratory epithelium the ciliated cells were labelled with WGA and s-WGA, while the secretory cells bound most of the lectins. Thus different sugars are exposed on the surface of the different types of epithelia in the nasal cavity, providing a basis for selectivity in microbial attacks on these areas.
对12日龄瑞士白化小鼠（NMRI品系）鼻腔的切片进行凝集素组织化学分析。一组生物素化凝集素（刀豆球蛋白A、小麦胚凝集素、大豆凝集素、花生凝集素、荆豆凝集素、双花扁豆凝集素和UEA I）以及一种辣根过氧化物酶偶联凝集素（GSA II）显示出与呼吸细胞和神经上皮细胞结合的显著差异。荆豆凝集素（对半乳糖和N-乙酰半乳糖胺有亲和力）、花生凝集素（对半乳糖）和小麦胚凝集素（对唾液酸和N-乙酰葡糖胺）标记了嗅觉和犁鼻器上皮中的受体神经元。双花扁豆凝集素（对N-乙酰半乳糖胺）标记了约5%的嗅觉受体神经元亚群，但标记了犁鼻器中的大多数神经元。UEA I（对岩藻糖）和大豆凝集素（对N-乙酰葡糖胺）强烈标记了犁鼻器中的整个神经细胞群体，但在嗅觉上皮中，这些凝集素的标记是分层的。在呼吸上皮中，纤毛细胞被小麦胚凝集素和大豆凝集素标记，而分泌细胞结合了大多数凝集素。因此，鼻腔中不同类型上皮细胞表面暴露有不同的糖类，为微生物侵袭这些区域时的选择性提供了基础。
