使用基于光子爆发计数技术的免疫传感器原型检测临床标本中的轮状病毒。
Detection of rotavirus in clinical specimens using an immunosensor prototype based on the photon burst counting technique.
作者信息
Hasegawa Makoto, Wandera Ernest Apondi, Inoue Yuka, Kimura Nanami, Sasaki Ryuzo, Mizukami Tamio, Shah Mohammad Monir, Shirai Nobuaki, Takei Osamu, Shindo Hironori, Ichinose Yoshio
机构信息
Graduate School of Bioscience, Nagahama Institute of Bioscience and Technology, 1266 Tamura, Nagahama-shi, Shiga 526-0829, Japan.
Kenya Research Station, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki-shi, Nagasaki 852-8523, Japan.
出版信息
Biomed Opt Express. 2017 Jun 23;8(7):3383-3394. doi: 10.1364/BOE.8.003383. eCollection 2017 Jul 1.
In this study, a sensitive fluorescence sensor was developed for the detection of small, fluorescence-labeled particles dispersed in a solution. The prototype system comprises of a laser confocal optical system and a mechanical sample stage to detect photon bursting of fluorescence-labeled small particles in sample volumes less than 5 μL within 3 minutes. To examine the feasibility of the prototype system as a diagnostic tool, assemblages of rotavirus and fluorescence-labeled antibody were analyzed. The detection sensitivity for rotavirus was 1 × 10 pfu/mL. Rotavirus in stool samples from patients with acute gastroenteritis was also detected. The advantages and disadvantages of this immunosensor with respect to ELISA and RT-PCR, the current gold standards for virus detection, are discussed.
在本研究中，开发了一种灵敏的荧光传感器，用于检测分散在溶液中的荧光标记小颗粒。该原型系统由激光共焦光学系统和机械样品台组成，可在3分钟内检测体积小于5 μL的样品中荧光标记小颗粒的光子爆发。为检验该原型系统作为诊断工具的可行性，对轮状病毒和荧光标记抗体的组合进行了分析。轮状病毒的检测灵敏度为1×10 pfu/mL。还检测了急性肠胃炎患者粪便样本中的轮状病毒。讨论了这种免疫传感器相对于病毒检测的当前金标准酶联免疫吸附测定（ELISA）和逆转录聚合酶链反应（RT-PCR）的优缺点。
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