Institute of Tropical Medicine, Center for Microbiology Research, KEMRI, KEMRI-Nagasaki University, Nairobi, Kenya.
Department of Virology and Parasitology, School of Medicine, Fujita Health University, Toyoake, Japan.
J Med Virol. 2017 May;89(5):809-817. doi: 10.1002/jmv.24691. Epub 2016 Sep 29.
Between July 2009 and June 2014, a total of 1,546 fecal specimens were collected from children <5 years of age with acute gastroenteritis admitted to Kiambu County Hospital, Central Kenya. The specimens were screened for group A rotavirus (RVA) using ELISA, and RVA-positive specimens were subjected to semi-nested RT-PCR to determine the G and P genotypes. RVA was detected in 429/1,546 (27.5%) fecal specimens. RVA infections occurred in all age groups <59 months, with an early peak at 6-17 months. The infections persisted year-round with distinct seasonal peaks depending on the year. G1P[8] (28%) was the most predominant genotype, followed by G9P[8] (12%), G8P[4] (7%), G1P[4] (5%), G9P[4] (4%), and G12P[6] (3%). In the yearly change of G and P genotypes, a major shift from G9P[8] to G1P[8] was found in 2012. Phylogenetic analysis of the nucleotide sequences of the VP7 and VP4 genes of seven strains with unusual G8 or P[6] showed that the VP7 nucleotide sequences of G8 were clustered in lineage 6 in which African strains are included, and that there are at least two distinct VP4 nucleotide sequences of P[6] strains. These results represent basic data on RVA strains circulating in this region before vaccine introduction. J. Med. Virol. 89:809-817, 2017. © 2016 Wiley Periodicals, Inc.
2009 年 7 月至 2014 年 6 月期间，从肯尼亚中部基安布县医院收治的急性肠胃炎 5 岁以下儿童中采集了 1546 份粪便标本。使用 ELISA 法对这些标本进行 A 组轮状病毒（RVA）筛查，对 RVA 阳性标本进行半巢式 RT-PCR 以确定 G 和 P 基因型。在 1546 份粪便标本中检测到 429 份 RVA。RVA 感染发生在所有<59 个月的年龄组中，6-17 个月时出现早期高峰。感染全年持续存在，每年都有明显的季节性高峰。G1P[8]（28%）是最主要的基因型，其次是 G9P[8]（12%）、G8P[4]（7%）、G1P[4]（5%）、G9P[4]（4%）和 G12P[6]（3%）。在 G 和 P 基因型的年度变化中，2012 年发现 G9P[8]向 G1P[8]的主要转变。7 株具有异常 G8 或 P[6]的 VP7 和 VP4 基因核苷酸序列的系统进化分析表明，G8 的 VP7 核苷酸序列聚类在包含非洲株的 6 谱系中，并且 P[6]株至少有两种不同的 VP4 核苷酸序列。这些结果代表了疫苗引入前该地区 RVA 株的基本数据。J. Med. Virol. 89:809-817, 2017. © 2016 Wiley Periodicals, Inc.
