Chitambar Shobha D, Tatte Vaishali S, Dhongde Ram, Kalrao Vijay
Rotavirus Group, National Institute of Virology, Pune, India.
J Med Virol. 2008 Dec;80(12):2169-76. doi: 10.1002/jmv.21338.
Recently, rotavirus antigenemia and viremia have been identified in patients with acute gastroenteritis. This study examined rotavirus viremia in children hospitalized for acute gastroenteritis in order to establish its association with fecal shedding of rotavirus, infecting genotypes and antibody marker of acute infection. Thirty-one pairs of stool-serum specimens were collected from November 2004 to February 2005 together with clinical information. All paired specimens were screened for rotavirus RNA by RT-PCR using the VP6 gene primers. All stool and serum specimens were tested for rotavirus antigen and anti-rotavirus IgM respectively by ELISA. Sixteen of 31 stool-serum pairs showed the presence of rotavirus RNA. Nine stool and two serum specimens were positive only by RT-PCR. The total positivity in rotavirus RNA was significantly higher in both stools (80.6%) and sera (58.1%) than that of stool antigen (38.7%) and anti-rotavirus IgM (25.8%) (P < 0.01). All PCR positive paired specimens were typed for the VP7 (G) and VP4 (P) genes. Five of sixteen pairs could be typed for both genes. Three of the five pairs showed concordance (G2P[4]/G2P[4]) while two showed discordance (G12P[8]/G2P[4], G8P[4]/G2P[4]) in the genotypes detected in stool and serum specimens respectively. The study documents a high frequency of rotavirus viremia in patients with acute diarrhea. The discordance of rotavirus strains at the genotypic level in the serum and stool of individual patients with diarrhea suggests the susceptibility of extra-intestinal sites for rotavirus infection and the possibility of differential dissemination of rotavirus strains from the intestine.
最近，在急性胃肠炎患者中已发现轮状病毒血症和病毒血症。本研究对因急性胃肠炎住院的儿童进行了轮状病毒血症检查，以确定其与轮状病毒粪便排出、感染基因型及急性感染抗体标志物之间的关联。2004年11月至2005年2月期间收集了31对粪便-血清标本及临床信息。使用VP6基因引物通过RT-PCR对所有配对标本进行轮状病毒RNA筛查。分别通过ELISA对所有粪便和血清标本检测轮状病毒抗原和抗轮状病毒IgM。31对粪便-血清标本中有16对显示存在轮状病毒RNA。9份粪便标本和2份血清标本仅通过RT-PCR呈阳性。轮状病毒RNA在粪便（80.6%）和血清（58.1%）中的总阳性率显著高于粪便抗原（38.7%）和抗轮状病毒IgM（25.8%）（P<0.01）。对所有PCR阳性的配对标本进行VP7（G）和VP4（P）基因分型。16对中有5对可对两个基因进行分型。5对中的3对显示一致（G2P[4]/G2P[4]），而另外2对在粪便和血清标本中检测到的基因型显示不一致（G12P[8]/G2P[4]，G8P[4]/G2P[4]）。该研究记录了急性腹泻患者中轮状病毒血症的高发生率。腹泻个体患者血清和粪便中轮状病毒株在基因型水平的不一致表明轮状病毒感染肠外部位的易感性以及轮状病毒株从肠道差异传播的可能性。
