Jin Xiao-Ling, Huang Ning, Shang Hui, Zhou Ming-Cheng, Hong Yi, Cai Wen-Zheng, Huang Jie
Department of Clinical Laboratory, The First Rehabilitation Hospital of Shanghai, Shanghai, China.
Department of Clinical Laboratory, Shandong Province Hospital of Traditional Chinese Medicine, Jinan, China.
J Clin Lab Anal. 2018 Mar;32(3). doi: 10.1002/jcla.22295. Epub 2017 Jul 18.
Our study was to explore the roles between serum soluble suppression of tumorigenicity 2 (sST2) and N-terminal pro-brain natriuretic peptide (NT-proBNP) while evaluating ventricular function to properly diagnose chronic heart failure (CHF).
In total, 197 CHF patients were recruited and classified into ventricular function's II, III, and IV groups, and 106 healthy people into normal control group. To detect concentrations of Sst2 and NT-proBNP, ELISA and electro-chemiluminescence immuno assay were implemented. An automatic biochemical analyzer was used to determine the levels of the following: blood urea nitrogen (BUN), creatinine (Cr), alanine aminotransferase (ALT), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), and uric acid (UA). A receiver operating characteristic (ROC) curve was adopted to detect the diagnostic value sST2 and NT-ProBNP in CHF and the logistic regression analysis involving the risk factors of CHF.
Serum sST2 and NT-proBNP concentrations were increased significantly in the ventricular function's II, III, and IV groups in a manner dependent on concentration as opposed to the manner the normal control group occupied. The area under the curve (AUC) of sST2, found NT-proBNP and sST2+NT-proBNP to be 0.942 (95% CI: 0.917-0.966), 0.920 (95% CI: 0.891-0.948), and 0.968 (95% CI: 0.953-0.984), respectively. sST2, NT-proBNP, UA, and Cr were verified as important risk factors of CHF.
Serum sST2 and NT-ProBNP could act as diagnostic indicators for CHF.
本研究旨在探讨血清可溶性肿瘤抑制因子2（sST2）与N端前脑钠肽（NT-proBNP）之间的关系，同时评估心室功能，以准确诊断慢性心力衰竭（CHF）。
共招募197例CHF患者，分为心室功能II级、III级和IV级组，106例健康人作为正常对照组。采用酶联免疫吸附测定（ELISA）和电化学发光免疫分析法检测sST2和NT-proBNP浓度。使用自动生化分析仪测定以下指标水平：血尿素氮（BUN）、肌酐（Cr）、丙氨酸转氨酶（ALT）、甘油三酯（TG）、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）和尿酸（UA）。采用受试者工作特征（ROC）曲线检测sST2和NT-ProBNP对CHF的诊断价值，并对CHF的危险因素进行逻辑回归分析。
心室功能II级、III级和IV级组血清sST2和NT-proBNP浓度显著升高，且呈浓度依赖性，与正常对照组情况相反。sST2、NT-proBNP和sST2 + NT-proBNP的曲线下面积（AUC）分别为0.942（95%CI：0.917 - 0.966）、0.920（95%CI：0.891 - 0.948）和0.968（95%CI：0.953 - 0.984）。sST2、NT-proBNP、UA和Cr被证实为CHF的重要危险因素。
血清sST2和NT-ProBNP可作为CHF的诊断指标。
