Borràs Eva, Sabidó Eduard
Proteomics Unit, Centre de Regulació Genòmica, Barcelona Institute of Science and Technology, Barcelona, Spain.
Universitat Pompeu Fabra, Barcelona, Spain.
Proteomics. 2017 Sep;17(17-18). doi: 10.1002/pmic.201700180.
Targeted proteomics has gained significant popularity in mass spectrometry-based protein quantification as a method to detect proteins of interest with high sensitivity, quantitative accuracy and reproducibility. However, with the emergence of a wide variety of targeted proteomics methods, some of them with high-throughput capabilities, it is easy to overlook the essence of each method and to determine what makes each of them a targeted proteomics method. In this viewpoint, we revisit the main targeted proteomics methods and classify them in four categories differentiating those methods that perform targeted data acquisition from targeted data analysis, and those methods that are based on peptide ion data (MS1 targeted methods) from those that rely on the peptide fragments (MS2 targeted methods).
靶向蛋白质组学作为一种能够高灵敏度、定量准确且可重复地检测目标蛋白质的方法,在基于质谱的蛋白质定量分析中已大受欢迎。然而,随着各种各样靶向蛋白质组学方法的出现,其中一些还具备高通量能力,人们很容易忽略每种方法的本质,难以确定究竟是什么使它们成为靶向蛋白质组学方法。基于这一观点,我们重新审视了主要的靶向蛋白质组学方法,并将它们分为四类,区分那些进行靶向数据采集的方法与进行靶向数据分析的方法,以及基于肽离子数据的方法(MS1靶向方法)与依赖肽片段的方法(MS2靶向方法)。