Bone marrow harvest from unrelated donors-up-to-date methodology.

OBJECTIVES

Bone marrow harvesting is one of the essential sources of stem cells for hematopoietic stem cell transplantation. We describe here the current "up-to-date" standard of the bone marrow harvest in unrelated stem cell donors.

METHODS

We analyzed medical data of 187 unrelated hematopoietic stem cell donors who underwent bone marrow harvest without previous peripheral blood stem collection at the center between 2011 and 2015. The methodology of marrow collection includes multiple cells aimed at safety of the procedure, for example, educational movie, modified skin disinfection protocol, cell enumeration during the procedure, reduction of the contamination surfaces, and ongoing monitoring of the quality of work of the doctors.

RESULTS

The total nucleated cell count over 2×10 per kg of recipient has been reached in 93.6% of harvests. All of the donors harvested more than 1×10 per kg of the recipient. There were no donors who required transfusions or had serious adverse events during and after the harvest.

CONCLUSION

We describe here the current up-to-date standard of bone marrow harvest, which leads to excellent results in majority of donors without causing significant complications during the donation.