Siqueira Franciele Maboni, Pérez-Wohlfeil Esteban, Carvalho Fabíola Marques, Trelles Oswaldo, Schrank Irene Silveira, Vasconcelos Ana Tereza Ribeiro, Zaha Arnaldo
Centro de Biotecnologia, Universidade Federal de Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
Department of Computer Architecture, University of Malaga, Malaga, Spain.
PLoS One. 2017 Jul 18;12(7):e0181503. doi: 10.1371/journal.pone.0181503. eCollection 2017.
Mycoplasma hyopneumoniae is the etiologic agent of swine enzootic pneumonia. However other mycoplasma species and secondary bacteria are found as inhabitants of the swine respiratory tract, which can be also related to disease. In the present study we have performed a total DNA metagenomic analysis from the lungs of pigs kept in a field condition, with suggestive signals of enzootic pneumonia and without any infection signals to evaluate the bacteria variability of the lungs microbiota. Libraries from metagenomic DNA were prepared and sequenced using total DNA shotgun metagenomic pyrosequencing. The metagenomic distribution showed a great abundance of bacteria. The most common microbial families identified from pneumonic swine's lungs were Mycoplasmataceae, Flavobacteriaceae and Pasteurellaceae, whereas in the carrier swine's lungs the most common families were Mycoplasmataceae, Bradyrhizobiaceae and Flavobacteriaceae. Analysis of community composition in both samples confirmed the high prevalence of M. hyopneumoniae. Moreover, the carrier lungs had more diverse family population, which should be related to the lungs normal flora. In summary, we provide a wide view of the bacterial population from lungs with signals of enzootic pneumonia and lungs without signals of enzootic pneumonia in a field situation. These bacteria patterns provide information that may be important for the establishment of disease control measures and to give insights for further studies.
猪肺炎支原体是猪地方流行性肺炎的病原体。然而，其他支原体物种和继发性细菌也存在于猪的呼吸道中，它们也可能与疾病有关。在本研究中，我们对处于田间环境、有地方流行性肺炎暗示信号且无任何感染信号的猪的肺部进行了全DNA宏基因组分析，以评估肺部微生物群的细菌变异性。使用全DNA鸟枪法宏基因组焦磷酸测序制备并测序了宏基因组DNA文库。宏基因组分布显示细菌数量众多。从患肺炎猪的肺部鉴定出的最常见微生物科是支原体科、黄杆菌科和巴斯德氏菌科，而在携带猪的肺部，最常见的科是支原体科、慢生根瘤菌科和黄杆菌科。对两个样本中群落组成的分析证实了猪肺炎支原体的高流行率。此外，携带猪的肺部菌群更为多样，这可能与肺部正常菌群有关。总之，我们全面了解了田间环境下有地方流行性肺炎信号的肺部和无地方流行性肺炎信号的肺部的细菌种群情况。这些细菌模式提供的信息可能对制定疾病控制措施很重要，并为进一步研究提供见解。
