The School of Chemistry & Chemical Engineering, State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, Shanghai 200240, China.
State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Colloids Surf B Biointerfaces. 2017 Oct 1;158:408-415. doi: 10.1016/j.colsurfb.2017.07.020. Epub 2017 Jul 8.
Biosynthesis of nanoparticles from plant extracts is receiving enormous interest due to their abundant availability and a broad spectrum of bioactive reducing metabolites. In this study, the reducing potential of Artemisia vulgaris leaves extract (AVLE) was investigated for synthesizing silver nanoparticles without the addition of any external reducing or capping agent. The appearance of blackish brown color evidenced the complete synthesis of nanoparticles. The synthesized silver nanoparticles were characterized by UV-vis spectroscopy, scanning electron microscope (SEM), energy dispersive X-ray spectroscopy (EDX), transmission electron microscope (TEM), atomic force microscopy (AFM) and Fourier transforms infrared spectroscopy (FT-IR) analysis. UV-vis absorption profile of the bio-reduced sample elucidated the main peak around 420nm, which correspond to the surface plasmon resonance of silver nanoparticles. SEM and AFM analyses confirmed the morphology of the synthesized nanoparticles. Similarly, particles with a distinctive peak of silver were examined with EDX. The average diameter of silver nanoparticles was about 25nm from Transmission Electron Microscopy (TEM). FTIR spectroscopy scrutinized the involvement of various functional groups during nanoparticle synthesis. The green synthesized nanoparticles presented effective antibacterial activity against pathogenic bacteria than AVLE alone. In-vitro antioxidant assays revealed that silver nanoparticles (AV-AgNPs) exhibited promising antioxidant properties. The nanoparticles also displayed a potent cytotoxic effect against HeLa and MCF-7 cell lines. In conclusion, the results supported the advantages of employing a bio-green approach for developing silver nanoparticles with antimicrobial, antioxidant, and antiproliferative activities in a simple and cost- competitive manner.
由于植物提取物来源丰富，且含有多种生物活性还原代谢物，因此利用植物提取物合成纳米颗粒受到了广泛关注。本研究旨在探讨艾蒿叶提取物（AVLE）在无外加还原剂或稳定剂的条件下还原合成银纳米颗粒的能力。黑褐色表明纳米颗粒已完全合成。通过紫外可见分光光度法、扫描电子显微镜（SEM）、能谱（EDX）、透射电子显微镜（TEM）、原子力显微镜（AFM）和傅里叶变换红外光谱（FT-IR）分析对合成的银纳米颗粒进行了表征。生物还原样品的紫外-可见吸收光谱阐明了主要峰值在 420nm 左右，这对应于银纳米颗粒的表面等离子体共振。SEM 和 AFM 分析证实了合成纳米颗粒的形态。同样，EDX 检测到具有明显银峰的颗粒。透射电子显微镜（TEM）测量的银纳米颗粒平均直径约为 25nm。傅里叶变换红外光谱（FTIR）分析表明，在纳米颗粒合成过程中涉及到各种功能基团。与 AVLE 相比，绿色合成的纳米颗粒对致病菌表现出更强的抗菌活性。体外抗氧化分析表明，银纳米颗粒（AV-AgNPs）具有良好的抗氧化性能。这些纳米颗粒对 HeLa 和 MCF-7 细胞系也表现出很强的细胞毒性。综上所述，这些结果支持采用生物绿色方法制备具有抗菌、抗氧化和抗增殖活性的银纳米颗粒的优势，且这种方法简单、经济高效。
