Nath Sunayana, Shyanti Ritis Kumar, Singh Rana Pratap, Mishra Manoj, Pathak Bhawana
School of Environment and Sustainable Development, Central University of Gujarat, Gandhinagar, Gujarat, India.
School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
Front Microbiol. 2024 Jan 5;14:1324111. doi: 10.3389/fmicb.2023.1324111. eCollection 2023.
The present study investigated the synthesis and biological applications of green, economical, and multifunctional silver and gold nanoparticles (TSAgNPs and TSAuNPs) using the ethnomedical important medicinal plant for biological activities. Relatively higher levels of antioxidant components were measured in compared to the well-known , and suggested the potential of for the study. Synthesized TSAgNPs and TSAuNPs were characterized through UV-Vis, XRD, SEM-EDS, HR-TEM, SAED, and FTIR techniques. SEM revealed that TSAgNPs and TSAuNPs were predominantly spherical in shape with 19 ± 7.3 and 43 ± 6.3 nm crystal sizes. The sizes of TSAgNPs and TSAuNPs were found to be12 ± 4.8 and 45 ± 2.9 nm, respectively, according to TEM measurements. The FTIR and phytochemical analyses revealed that the polyphenols and proteins present in may act as bio-reducing and stabilizing agents for the synthesis. Synthesized NPs exhibited enhanced scavenging properties for ABTS and DPPH radicals. TSAgNPs and TSAuNPs were able to protect DNA nicking up to 13.48% and 15.38%, respectively, from oxidative stress. TSAgNPs possessed efficient antibacterial activities in a concentration-dependent manner against human pathogenic bacteria, such as , , and . Furthermore, TSAgNPs and TSAuNPs showed significant cytotoxicity against FaDu HNSCC grown in 2D at 50 and 100 μg mL. Tumor inhibitory effects on FaDu-derived spheroid were significant for TSAgNPs > TSAuNPs at 100 μg mL in 3D conditions. Dead cells were highest largely for TSAgNPs (76.65% ± 1.76%), while TSAuNPs were non-significant, and Saq was ineffectively compared with the control. However, the diameter of the spheroid drastically reduced for TSAgNPs (3.94 folds) followed by TSAuNPs (2.58 folds), Saq (1.94 folds), and cisplatin (1.83 folds) at 100 μg mL. The findings of the study suggested the bio-competence of TSAgNPs and TSAuNPs as multi-responsive agents for antioxidants, DNA protection, antibacterial, and anti-tumor activities to provide a better comprehension of the role of phytogenic nanoparticles in healthcare systems.
本研究利用具有民族医学重要性的药用植物研究了绿色、经济且多功能的银和金纳米颗粒（TSAgNPs和TSAuNPs）的合成及其生物应用，以用于生物活性研究。与著名的[植物名称未给出]相比，[研究中植物名称未给出]中测得的抗氧化成分水平相对较高，这表明[该植物]在该研究中的潜力。通过紫外可见光谱、X射线衍射、扫描电子显微镜 - 能谱分析、高分辨率透射电子显微镜、选区电子衍射和傅里叶变换红外光谱技术对合成的TSAgNPs和TSAuNPs进行了表征。扫描电子显微镜显示，TSAgNPs和TSAuNPs主要呈球形，晶体尺寸分别为19±7.3和43±6.3纳米。根据透射电子显微镜测量，TSAgNPs和TSAuNPs的尺寸分别为12±4.8和45±2.9纳米。傅里叶变换红外光谱和植物化学分析表明，[植物名称未给出]中存在的多酚和蛋白质可能作为合成的生物还原剂和稳定剂。合成的纳米颗粒对ABTS和DPPH自由基表现出增强的清除特性。TSAgNPs和TSAuNPs能够分别将DNA切口从氧化应激中保护至13.48%和15.38%。TSAgNPs对人类病原菌如[细菌名称未给出]、[细菌名称未给出]、[细菌名称未给出]和[细菌名称未给出]具有浓度依赖性的高效抗菌活性。此外，TSAgNPs和TSAuNPs在二维培养条件下，对FaDu头颈部鳞状细胞癌在50和100μg/mL时表现出显著的细胞毒性。在三维条件下，100μg/mL时，TSAgNPs对FaDu来源的球体的肿瘤抑制作用显著大于TSAuNPs。TSAgNPs导致的死细胞数量最高（76.65%±1.76%），而TSAuNPs则不显著，与对照相比，[提取物名称未给出]无效。然而，在100μg/mL时，TSAgNPs使球体直径急剧减小（3.94倍），其次是TSAuNPs（2.58倍）、[提取物名称未给出]（1.94倍）和顺铂（1.83倍）。该研究结果表明，TSAgNPs和TSAuNPs作为抗氧化剂、DNA保护、抗菌和抗肿瘤活性的多响应剂具有生物活性，有助于更好地理解植物源纳米颗粒在医疗保健系统中的作用。
