Blaine E H, Seymour A A, Marsh E A, Napier M A
Fed Proc. 1986 Jun;45(7):2122-7.
Anesthetized beagle dogs received increasing doses of continuous infusions of a 26-amino-acid synthetic atrial natriuretic factor (ANF). Urinary sodium excretion rose in a dose-dependent manner to a maximum level similar to that seen after hydrochlorothiazide administration. Mean arterial blood pressure decreased, but only modestly, and not in a dose-dependent fashion. Dogs chronically retaining NaCl secondary to constriction of the thoracic inferior vena cava showed only modestly enhanced natriuresis when infused with similar levels of ANF. When ANF was infused directly into the renal artery of anesthetized beagles, a dose-dependent natriuresis and calciuresis were observed with maximal fractional sodium excretion averaging approximately 8%. Although glomerular filtration tended to increase, the average dose-related changes were not significant. Cyclic GMP excretion was increased during intra-renal-arterial infusion of ANF. Excretion of cyclic GMP by both the infused and noninfused kidneys was equal, which suggests that urinary cyclic GMP was not nephrogenous but derived from the elevated circulating levels. These and other data from rats dissociate changes in urinary cyclic GMP excretion and sodium excretion.
对麻醉的比格犬持续输注递增剂量的一种含26个氨基酸的合成心房利钠因子（ANF）。尿钠排泄呈剂量依赖性增加，直至达到与给予氢氯噻嗪后所见相似的最高水平。平均动脉血压下降，但降幅较小，且不呈剂量依赖性。因胸段下腔静脉缩窄而长期潴留氯化钠的犬，在输注相似水平的ANF时，仅出现适度增强的利钠作用。当将ANF直接输注到麻醉比格犬的肾动脉中时，观察到剂量依赖性的利钠和利尿作用，最大钠排泄分数平均约为8%。尽管肾小球滤过率有增加的趋势，但与剂量相关的平均变化并不显著。在肾动脉内输注ANF期间，环磷酸鸟苷（cGMP）排泄增加。输注侧肾脏和未输注侧肾脏的cGMP排泄量相等，这表明尿cGMP并非肾源性，而是来源于循环水平的升高。来自大鼠的这些及其他数据表明，尿cGMP排泄变化与钠排泄变化无关。
