Chotiko Arranee, Sathivel Subramaniam
Division of Biology, Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi, Pathumthani, 12110 Thailand.
Department of Biological and Agricultural Engineering, Louisiana State University Agricultural Center, Baton Rouge, LA 70803 USA.
J Food Sci Technol. 2017 Jun;54(7):2059-2066. doi: 10.1007/s13197-017-2643-3. Epub 2017 May 6.
This study investigated pectin-based capsules as delivery systems for purple rice bran anthocyanin extract (AE) during exposure to simulated gastrointestinal conditions. Four different capsules loaded with AE were prepared by ionotropic gelation/extrusion, including (1) pectin capsules (PE), (2) pectin capsules coated with zein (PE/ZE), (3) pectin-whey protein isolate complex capsules (PE + WP), and (4) pectin-whey protein isolate complex capsules coated with zein (PE + WP/ZE). CaCl in an ethanol solution with or without zein was used as a crosslinking solution. Swelling and release characteristics of all capsules under simulated gastric fluid at pH 1.2 (SGF) and simulated intestinal fluid at pH 6.8 (SIF) for 120 and 180 min, respectively, were examined. PE + WP, PE + WP/ZE, and PE/ZE capsules had higher encapsulation efficiency than PE capsules. After incubation, PE + WP/ZE and PE capsules had the lowest swelling ratio in SGF and SIF, respectively. PE + WP/ZE capsules had the lowest AE release in SGF, while PE capsules had the highest. Both PE + WP and PE + WP/ZE capsules had significantly lower AE release in SIF than PE and PE/ZE capsules. The study demonstrated that PE + WP and PE + WP/ZE capsules have potential to function as a slow release delivery system for AE.
本研究考察了基于果胶的胶囊作为紫米糠花青素提取物（AE）在模拟胃肠道条件下的递送系统。通过离子凝胶化/挤压法制备了四种不同的负载AE的胶囊，包括（1）果胶胶囊（PE）、（2）用玉米醇溶蛋白包衣的果胶胶囊（PE/ZE）、（3）果胶-乳清蛋白分离物复合胶囊（PE + WP）和（4）用玉米醇溶蛋白包衣的果胶-乳清蛋白分离物复合胶囊（PE + WP/ZE）。含或不含玉米醇溶蛋白的乙醇溶液中的氯化钙用作交联溶液。分别考察了所有胶囊在pH 1.2的模拟胃液（SGF）和pH 6.8的模拟肠液（SIF）中120分钟和180分钟的溶胀和释放特性。PE + WP、PE + WP/ZE和PE/ZE胶囊的包封率高于PE胶囊。孵育后，PE + WP/ZE和PE胶囊分别在SGF和SIF中具有最低的溶胀率。PE + WP/ZE胶囊在SGF中的AE释放最低，而PE胶囊最高。PE + WP和PE + WP/ZE胶囊在SIF中的AE释放均显著低于PE和PE/ZE胶囊。该研究表明，PE + WP和PE + WP/ZE胶囊有潜力作为AE的缓释递送系统。
