Robinson A, Ashworth L A, Baskerville A, Irons L I
Dev Biol Stand. 1985;61:165-72.
Mice have been infected by intranasal instillation of Bordetella pertussis and the infection monitored by determining numbers of bacteria isolated from the lungs. Outer membrane proteins, filamentous hemagglutinin, toxoided-lymphocytosis promoting factor and agglutinogens (fimbriae) actively protect mice against intranasal infection and antibodies of the antigens neutralize infectivity. The neutralization of infection by agglutinins is serospecific. In general, antigens that actively protect mice against intracerebral infections also protect against intranasal infections but some antigens, such as filamentous hemagglutinin and agglutinogens, protect only against intranasal infections. The intranasal protective potency of antigens can be enhanced by including low levels of active lymphocytosis promoting factor in the preparations. The relevance of the intranasal test to the potency testing of pertussis vaccines is discussed.
通过鼻内滴注百日咳博德特氏菌感染小鼠，并通过测定从肺部分离出的细菌数量来监测感染情况。外膜蛋白、丝状血凝素、类毒素淋巴细胞增多促进因子和凝集原（菌毛）可有效保护小鼠免受鼻内感染，抗原的抗体可中和感染性。凝集素对感染的中和作用具有血清特异性。一般来说，能有效保护小鼠免受脑内感染的抗原也能预防鼻内感染，但有些抗原，如丝状血凝素和凝集原，仅能预防鼻内感染。通过在制剂中加入低水平的活性淋巴细胞增多促进因子，可以增强抗原的鼻内保护效力。本文还讨论了鼻内试验与百日咳疫苗效力测试的相关性。
