Bhatnagar R K, Ahmad S, Mukerji K G, Venkitasubramanian T A
J Appl Bacteriol. 1986 Mar;60(3):203-11. doi: 10.1111/j.1365-2672.1986.tb01074.x.
The relationship between nitrogen assimilation, metabolism and aflatoxin formation has been investigated in a toxigenic and a non-toxigenic strain of Aspergillus parasiticus. Ammonia from the medium is mainly assimilated via NADP-requiring glutamate dehydrogenase. During growth NAD-requiring glutamate dehydrogenase followed an inverse pattern of activity with respect to NADP glutamate dehydrogenase. Alpha-ketoglutarate, the product of NAD glutamate dehydrogenase, stimulated acetate incorporation into aflatoxins. Glutamine synthetase, ornithine transcarbamylase, both utilizing glutamate as substrate were assayed under different growth conditions. An important regulatory role for glutamine synthetase is suggested. The metabolic route of asparagine utilization was also investigated. Both the known pathways, glutamate oxaloacetate transaminase and glutamate pyruvate transaminase are operative simultaneously.
已对寄生曲霉的产毒菌株和非产毒菌株中氮同化、代谢与黄曲霉毒素形成之间的关系进行了研究。培养基中的氨主要通过需要NADP的谷氨酸脱氢酶进行同化。在生长过程中，需要NAD的谷氨酸脱氢酶的活性模式与需要NADP的谷氨酸脱氢酶相反。NAD谷氨酸脱氢酶的产物α-酮戊二酸刺激了乙酸盐掺入黄曲霉毒素中。在不同生长条件下测定了均以谷氨酸为底物的谷氨酰胺合成酶和鸟氨酸转氨甲酰酶。提示谷氨酰胺合成酶具有重要的调节作用。还研究了天冬酰胺利用的代谢途径。已知的两条途径，即谷氨酸草酰乙酸转氨酶和谷氨酸丙酮酸转氨酶同时发挥作用。
