Nord C E, Edlund C, Lahnborg G
J Antimicrob Chemother. 1986 Apr;17 Suppl B:59-63. doi: 10.1093/jac/17.suppl_b.59.
A reproducible experimental model of intra-abdominal infections in rats has been devised in order to stimulate intra-abdominal sepsis in patients. Pre-operatively, the rats were fed with lean ground beef for two weeks in order to change the intestinal flora to one similar to that of humans. A 1 cm segment of ileum was isolated on its vascular pedicle. The intestine was then divided at each end of the segment and intestinal continuity was re-established by an end-to-end anastomosis. The segment of ileum was then returned to the abdominal cavity. This experimental model was used to compare the efficacy of pefloxacin alone and in combination with metronidazole with the combination gentamicin and metronidazole in the treatment of intra-abdominal infections. Seventy per cent of the untreated animals died within two days. Within three days 40% of the animals receiving pefloxacin died. Animals treated with pefloxacin plus metronidazole or gentamicin plus metronidazole had a significantly decreased mortality and increased cure rates during the experimental period. Only 5% of these animals died. Thus the combination of pefloxacin and metronidazole seems to be as successful as gentamicin plus metronidazole in the treatment of intra-abdominal infections.
为了模拟人类腹腔感染，已设计出一种可重复的大鼠腹腔内感染实验模型。术前，给大鼠喂食瘦牛肉末两周，以便将肠道菌群改变为类似于人类的菌群。在回肠的血管蒂上分离出一段1厘米长的肠段。然后在该肠段的两端切断肠道，并通过端端吻合术重建肠道连续性。接着将回肠段放回腹腔。该实验模型用于比较单独使用培氟沙星以及培氟沙星与甲硝唑联合使用，与庆大霉素和甲硝唑联合使用在治疗腹腔内感染方面的疗效。70%的未治疗动物在两天内死亡。在三天内，接受培氟沙星治疗的动物中有40%死亡。在实验期间，接受培氟沙星加甲硝唑或庆大霉素加甲硝唑治疗的动物死亡率显著降低，治愈率提高。这些动物中只有5%死亡。因此，培氟沙星和甲硝唑联合使用在治疗腹腔内感染方面似乎与庆大霉素和甲硝唑联合使用一样成功。
