Department of Veterinary and Animal Sciences, University of Copenhagen, Copenhagen, Denmark.
Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Oslo, Norway.
Sci Rep. 2017 Jul 19;7(1):5930. doi: 10.1038/s41598-017-06324-7.
In an effort to reduce the frequency and severity of adverse reactions seen from the use of mineral oil adjuvants in salmonid fish, the effects of two alternative adjuvants were assessed, focusing on the induction of adverse effects as well as protection. Using rainbow trout (Oncorhynchus mykiss) as recipients, injection vaccines based on formalin-inactivated Aeromonas salmonicida subspecies salmonicida were formulated with CpG oligodeoxynucleotides, the liposomal cationic adjuvant formulation 01 (CAF01) or with Freund's incomplete adjuvant and administered intraperitoneally. Control groups of unvaccinated, Tris-buffered saline-injected or bacterin-injected individuals were included, and each group included in the study held a total number of 240 individuals. Subsequently, individuals from each group were examined for differences in Fulton's condition factor, macro- and microscopic pathological changes, as well as protection against experimental infection with A. salmonicida. While adverse effects were not eliminated, reductions in microscopic and macroscopic adverse effects, in particular, were seen for both the nucleotide- and liposome-based vaccine formulations. Furthermore, the induced protection appears similar to that of the benchmark formulation, thus introducing viable, potential alternative types of adjuvants for use in future fish vaccines.
为了减少鲑鱼科鱼类中矿物油佐剂使用引起的不良反应的频率和严重程度，评估了两种替代佐剂的效果，重点关注不良反应的诱导和保护作用。以虹鳟（Oncorhynchus mykiss）为受体，用CpG 寡脱氧核苷酸、脂质体阳离子佐剂制剂 01（CAF01）或弗氏不完全佐剂配制福尔马林灭活的鲑鱼气单胞菌亚种疫苗，并进行腹腔内注射。包括未接种疫苗、注射 Tris 缓冲盐水或菌苗的对照组，每组共 240 人。随后，对每组个体的富尔顿状况因子、宏观和微观病理变化以及对鲑鱼气单胞菌实验感染的保护作用进行检查。虽然不良反应并未消除，但核苷酸和脂质体疫苗制剂的微观和宏观不良反应均有所减少。此外，诱导的保护作用类似于基准制剂，因此为未来的鱼类疫苗引入了可行的、潜在的替代类型的佐剂。
