Suppr超能文献

饰纹姬蛙肺的转录组组装

transcriptome assembly for the lung of the ornamented pygmy frog ().

作者信息

Liu Lusha, Wang Shouhong, Zhao Lanying, Jiang Jianping

机构信息

Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China.

University of Chinese Academy of Sciences, Beijing 100049, China.

出版信息

Genom Data. 2017 Jul 4;13:44-45. doi: 10.1016/j.gdata.2017.07.002. eCollection 2017 Sep.

Abstract

, belonging to Neobatrachia, is a new model organism to study developmental biology, adaptive mechanisms from aquatic to terrestrial life, environmental toxicology, and human disease. use of lungs soon after hatching makes it extremely valuable for the study of lung function and development mechanisms. However, our knowledge of genes and pathways associated with lung development in is very limited. In this study, we conducted transcriptome assembly for the lung of using the Illumina HiSeq4000 platform. We obtained approximately 9.0 GB clean data from the lung of the stage 28 tadpole with lung inflation (NCBI accession numbers: SRP107055). transcriptome assembly identified 209,358 transcripts and 93,813 unigenes. In addition, BLASTX against NR, NT, KO, SwissProt, PFAM, GO and KOG databases were used to annotate all the 93,813 unigenes. This study provides the transcriptome and functional annotation of genes in lung development, which will be useful for comparative transcriptome analyses and promote research into mechanism of lung development in anuran.

摘要

属于新蛙亚目,是研究发育生物学、从水生到陆生生活的适应机制、环境毒理学和人类疾病的新型模式生物。孵化后不久就使用肺部,这使其对于肺功能和发育机制的研究极具价值。然而,我们对与肺部发育相关的基因和通路的了解非常有限。在本研究中,我们使用Illumina HiSeq4000平台对的肺进行转录组组装。我们从处于28期肺部充气的蝌蚪的肺中获得了约9.0GB的干净数据(NCBI登录号:SRP107055)。转录组组装鉴定出209,358个转录本和93,813个单基因。此外,使用针对NR、NT、KO、SwissProt、PFAM、GO和KOG数据库的BLASTX对所有93,813个单基因进行注释。本研究提供了肺部发育中基因的转录组和功能注释,这将有助于比较转录组分析,并促进对无尾两栖类动物肺部发育机制的研究。

相似文献

1
transcriptome assembly for the lung of the ornamented pygmy frog ().
Genom Data. 2017 Jul 4;13:44-45. doi: 10.1016/j.gdata.2017.07.002. eCollection 2017 Sep.
2
Gene Expression Program Underlying Tail Resorption During Thyroid Hormone-Dependent Metamorphosis of the Ornamented Pygmy Frog .
Front Endocrinol (Lausanne). 2019 Jan 25;10:11. doi: 10.3389/fendo.2019.00011. eCollection 2019.
5
Transcriptome profiling reveals gene regulation programs underlying tail development in the Ornamented Pygmy frog .
Front Biosci (Landmark Ed). 2021 Nov 30;26(11):1001-1012. doi: 10.52586/5004.
6
De Novo Assembly and Characterization of the Transcriptome of Grasshopper Shirakiacris shirakii.
Int J Mol Sci. 2016 Jul 22;17(7):1110. doi: 10.3390/ijms17071110.
9
Characterization and analysis of a de novo transcriptome from the pygmy grasshopper Tetrix japonica.
Mol Ecol Resour. 2017 May;17(3):381-392. doi: 10.1111/1755-0998.12553. Epub 2016 Jul 20.
10
The complete mitogenome of (Anura: Microhylidae) and phylogenetic analysis using GenBank data mining.
Mitochondrial DNA B Resour. 2019 Sep 18;4(2):3049-3050. doi: 10.1080/23802359.2019.1666670.

引用本文的文献

本文引用的文献

1
Research proceedings on amphibian model organisms.
Dongwuxue Yanjiu. 2016 Jul 18;37(4):237-45. doi: 10.13918/j.issn.2095-8137.2016.4.237.
2
Plasticity of lung development in the amphibian, Xenopus laevis.
Biol Open. 2013 Dec 15;2(12):1324-35. doi: 10.1242/bio.20133772.
3
Full-length transcriptome assembly from RNA-Seq data without a reference genome.
Nat Biotechnol. 2011 May 15;29(7):644-52. doi: 10.1038/nbt.1883.

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验