Liu Lusha, Wang Shouhong, Zhao Lanying, Jiang Jianping
Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
Genom Data. 2017 Jul 4;13:44-45. doi: 10.1016/j.gdata.2017.07.002. eCollection 2017 Sep.
, belonging to Neobatrachia, is a new model organism to study developmental biology, adaptive mechanisms from aquatic to terrestrial life, environmental toxicology, and human disease. use of lungs soon after hatching makes it extremely valuable for the study of lung function and development mechanisms. However, our knowledge of genes and pathways associated with lung development in is very limited. In this study, we conducted transcriptome assembly for the lung of using the Illumina HiSeq4000 platform. We obtained approximately 9.0 GB clean data from the lung of the stage 28 tadpole with lung inflation (NCBI accession numbers: SRP107055). transcriptome assembly identified 209,358 transcripts and 93,813 unigenes. In addition, BLASTX against NR, NT, KO, SwissProt, PFAM, GO and KOG databases were used to annotate all the 93,813 unigenes. This study provides the transcriptome and functional annotation of genes in lung development, which will be useful for comparative transcriptome analyses and promote research into mechanism of lung development in anuran.
属于新蛙亚目,是研究发育生物学、从水生到陆生生活的适应机制、环境毒理学和人类疾病的新型模式生物。孵化后不久就使用肺部,这使其对于肺功能和发育机制的研究极具价值。然而,我们对与肺部发育相关的基因和通路的了解非常有限。在本研究中,我们使用Illumina HiSeq4000平台对的肺进行转录组组装。我们从处于28期肺部充气的蝌蚪的肺中获得了约9.0GB的干净数据(NCBI登录号:SRP107055)。转录组组装鉴定出209,358个转录本和93,813个单基因。此外,使用针对NR、NT、KO、SwissProt、PFAM、GO和KOG数据库的BLASTX对所有93,813个单基因进行注释。本研究提供了肺部发育中基因的转录组和功能注释,这将有助于比较转录组分析,并促进对无尾两栖类动物肺部发育机制的研究。