Shramova E I, Proshkina G M, Deyev S M, Petrov R V
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia.
Lobachevsky Nizhny Novgorod State University, Nizhny Novgorod, 603950, Russia.
Dokl Biochem Biophys. 2017 May;474(1):228-230. doi: 10.1134/S160767291703019X. Epub 2017 Jul 20.
It is proposed to use the bioluminescent resonance energy transfer to solve the problem of creating the internal light sources in photodynamic therapy of cancer. Energy donor in the developed system is the oxidized form of the luciferase NanoLuc substrate furimamide, and acceptor is the phototoxic fluorescent protein miniSOG. It is shown that, in the proposed system, the photoinduced cytotoxicity of flavoprotein miniSOG in vitro depends on the intracellular localization, and the cytotoxic effect is 48% for the cytoplasmic localization of the fusion protein, 65% for the mitochondrial localization, and 69% for the membrane localization. The obtained data indicate that, for maximization of the photodynamic effect in vivo, it is appropriate to use the NanoLuc-miniSOG fusion protein in the membrane localization.
有人提议利用生物发光共振能量转移来解决癌症光动力治疗中创建内部光源的问题。在已开发的系统中，能量供体是荧光素酶NanoLuc底物呋酰胺的氧化形式，受体是光毒性荧光蛋白miniSOG。结果表明，在所提出的系统中，黄素蛋白miniSOG在体外的光诱导细胞毒性取决于细胞内定位，融合蛋白定位于细胞质时细胞毒性效应为48%，定位于线粒体时为65%，定位于膜时为69%。所获得的数据表明，为了在体内使光动力效应最大化，宜使用定位于膜的NanoLuc-miniSOG融合蛋白。
