Shramova E I, Proshkina G M, Chumakov S P, Khodarovich Yu M, Deyev S M
Shemyakin/Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, 117997, Moscow, Russia.
Shemyakin/Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, 117997, Moscow, Russia ; National Research Tomsk Polytechnic University, Lenina av., 30, 634050, Tomsk, Russia.
Acta Naturae. 2016 Oct-Dec;8(4):118-123.
In this study, we investigated the possibility of phototoxic flavoprotein miniSOG (photosensitizer) excitation in cancer cells by bioluminescence occurring when luciferase NanoLuc oxidizes its substrate, furimazine. We have shown that the phototoxic flavoprotein miniSOG expressed in eukaryotic cells in fusion with NanoLuc luciferase is activated in the presence of its substrate, furimazine. Upon such condition, miniSOG possesses photoinduced cytotoxicity and causes a 48% cell death level in a stably transfected cell line.
在本研究中，我们探究了癌细胞中光毒性黄素蛋白miniSOG（光敏剂）被荧光素酶NanoLuc氧化其底物腔肠素时产生的生物发光激发的可能性。我们已经表明，与NanoLuc荧光素酶融合在真核细胞中表达的光毒性黄素蛋白miniSOG在其底物腔肠素存在时被激活。在这种情况下，miniSOG具有光诱导的细胞毒性，并在稳定转染的细胞系中导致48%的细胞死亡水平。
