Department of Plant Pathology, North Dakota State University, Fargo, ND 58102, USA.
USDA-ARS, Red River Valley Agricultural Resarch Center, Cereal Crops Research Unit, Fargo, ND 58102, USA.
Fungal Genet Biol. 2017 Oct;107:12-19. doi: 10.1016/j.fgb.2017.07.003. Epub 2017 Jul 17.
Pyrenophora teres f. teres is the causal agent of net form net blotch (NFNB) of barley. In order to map the genetics of avirulence/virulence in P. teres f. teres, a fungal population was developed using P. teres f. teres isolates BB25 (Denmark) and FGOH04Ptt-21 (North Dakota, USA) due to these two isolates differing in virulence on several common barley lines. 109 progeny isolates were obtained from the BB25 by FGOH04Ptt-21 cross that were then used for NFNB disease evaluation across eight barley lines, four of which have been used commonly as NFNB differential lines as well as four cultivars commonly used in barley production in the Northern Great Plains. BB25 was virulent on one of the barley lines and avirulent on seven of the barley lines whereas, FGOH04Ptt-21 was virulent on all eight barley lines evaluated. Genetic maps were generated with single nucleotide polymorphism (SNP) markers obtained using a restriction associated DNA genotyping by sequencing (RAD-GBS) approach. Sixteen linkage groups were formed and were used to identify quantitative trait loci (QTL) associated with avirulence/virulence. Nine unique QTL were identified on eight linkage groups out of which three QTL had major effects (R≥45%) while the remaining six QTL were relatively minor (R<20%). One or two major effect loci were identified for the lines commonly used as differentials. Conversely, variation in virulence on the local barley cultivars was mostly associated with small effect loci that contributed quantitatively to disease.
层出镰刀菌（Pyrenophora teres f. teres）是大麦网斑病（NFNB）的致病菌。为了对层出镰刀菌（Pyrenophora teres f. teres）的无毒/毒性进行遗传作图，利用丹麦的 BB25 菌株和美国北达科他州的 FGOH04Ptt-21 菌株开发了一个真菌种群，因为这两个菌株在几种常见的大麦品系上的毒性不同。从 BB25 到 FGOH04Ptt-21 的杂交中获得了 109 个后代分离株，然后用于在 8 个大麦品系上进行 NFNB 疾病评估，其中 4 个品系通常作为 NFNB 差异品系使用，另外 4 个是大平原北部大麦生产中常用的品种。BB25 在一个大麦品系上是毒性的，而在 7 个大麦品系上是无毒的，而 FGOH04Ptt-21 在所有 8 个评估的大麦品系上都是毒性的。使用基于限制性相关 DNA 测序的基因型（RAD-GBS）方法获得的单核苷酸多态性（SNP）标记生成了遗传图谱。形成了 16 个连锁群，并用于鉴定与无毒/毒性相关的数量性状基因座（QTL）。在 8 个连锁群中鉴定出了 9 个独特的 QTL，其中 3 个 QTL 具有主要效应（R≥45%），而其余 6 个 QTL 则相对较小（R<20%）。通常作为差异品系使用的品系有一个或两个主要效应位点。相反，当地大麦品种上的毒性变化主要与小效应位点相关，这些位点对疾病有定量贡献。
