Koladia V M, Faris J D, Richards J K, Brueggeman R S, Chao S, Friesen T L
Department of Plant Pathology, North Dakota State University, Fargo, ND, USA.
Cereal Crops Research Unit, Northern Crop Science Laboratory, USDA-ARS, Fargo, ND, USA.
Theor Appl Genet. 2017 Jan;130(1):163-173. doi: 10.1007/s00122-016-2801-4. Epub 2016 Oct 12.
A CIho 5791 × Tifang recombinant inbred mapping population was developed and used to identify major dominant resistance genes on barley chromosomes 6H and 3H in CI5791 and on 3H in Tifang. The barley line CIho 5791 confers high levels of resistance to Pyrenophora teres f. teres, causal agent of net form net blotch (NFNB), with few documented isolates overcoming this resistance. Tifang barley also harbors resistance to P. teres f. teres which was previously shown to localize to barley chromosome 3H. A CIho 5791 × Tifang F recombinant inbred line (RIL) population was developed using single seed descent. The Illumina iSelect SNP platform was used to identify 2562 single nucleotide polymorphism (SNP) markers across the barley genome, resulting in seven linkage maps, one for each barley chromosome. The CIho 5791 × Tifang RIL population was evaluated for NFNB resistance using nine P. teres f. teres isolates collected globally. Tifang was resistant to four of the isolates tested whereas CIho 5791 was highly resistant to all nine isolates. QTL analysis indicated that the CIho 5791 resistance mapped to chromosome 6H whereas the Tifang resistance mapped to chromosome 3H. Additionally, CIho 5791 also harbored resistance to two Japanese isolates that mapped to a 3H region similar to that of Tifang. SNP markers and RILs harboring both 3H and 6H resistance will be useful in resistance breeding against NFNB.
构建了一个CIho 5791×提方重组自交系定位群体，并用于鉴定CI5791大麦6H和3H染色体以及提方3H染色体上的主要显性抗性基因。大麦品系CIho 5791对网斑病病原菌网形网斑病菌（NFNB）具有高水平抗性，几乎没有文献记载有分离株能克服这种抗性。提方大麦也对网形网斑病菌具有抗性，此前已证明该抗性定位于大麦3H染色体。利用单粒传法构建了CIho 5791×提方F重组自交系（RIL）群体。使用Illumina iSelect SNP平台在大麦全基因组中鉴定了2562个单核苷酸多态性（SNP）标记，从而得到七张连锁图谱，每个大麦染色体一张。利用全球收集的9个网形网斑病菌分离株对CIho 5791×提方RIL群体进行了NFNB抗性评估。提方对4个测试分离株具有抗性，而CIho 5791对所有9个分离株都具有高抗性。QTL分析表明，CIho 5791的抗性定位于染色体上，而提方的抗性定位于3H染色体。此外，CIho 5791对两个日本分离株也具有抗性，这两个分离株的抗性定位于与提方相似的3H区域。携带3H和6H抗性的SNP标记和RILs将有助于抗NFNB的抗性育种。
